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对中国南海的软珊瑚Scleronephthyasp .的化学成分进行研究 ,从有免疫调节活性的乙醚可溶物中分离鉴定了 13个化合物 ,经1HNMR ,13 CNMR ,HMQC ,HMBC ,1H 1HCOSY和MS等光谱分析并结合文献数据 ,确证其结构分别为 :3 β 羟基 2 0 烯孕甾烷乙酸酯 ( 1) ,3 β 羟基 5 ,2 0 二烯孕甾烷乙酸酯 ( 2 ) ,3 酮 2 0 烯孕甾烷 ( 3 ) ,1 ,2 环氧 3 酮 2 0 烯孕甾烷( 4 ) ,1 ,2 0 二烯 3 酮孕甾烷 ( 5 ) ,1 烯 3 酮 2 0S 甲基 2 1 羟基孕甾烷乙酸酯 ( 6) ,1 烯 3 酮 2 0 β 羟基孕甾烷乙酸酯( 7) ,1 烯 3 ,2 2 二酮胆甾烷 ( 8) ,1 ,2 4 二烯 3 酮 2 2 ,2 3 二羟基胆甾烷 ( 9) ,O methylisogrifolin ( 1 0 ) ,( 1R ,3S,4S,7E ,1 1E) 3α,4α 环氧 7,1 1 ,1 5 三烯西松烷 ( 1 1 ) ,玉米黄素 1 2和 6 溴 3 羧酸吲哚 ( 1 3 ) ,其中化合物 4,6和 1 0是首次从自然界分离得到 .首次对化合物 6,7和 1 0的波谱数据进行了报道 ,而且利用 2DNMR对其进行了全归属 .对化合物 1～7可能的生源关系也进行了探讨 .化合物 6和 7对人宫颈癌细胞 (HELA)有抑制活性 ,IC50 分别为 3 .3和6.3mol/L ;化合物7对肝癌细胞 (BE740 2 )和人白血病细胞 (HL 60 )也显示抑制活性 ,IC50 分别为 3 .1和 0 .2 6mol/L ;化合物11对CO
The chemical constituents of Scleronephydasp., A soft coral in the South China Sea, were studied. Thirteen compounds were isolated and identified from immunosuppressive ether solubles and analyzed by 1H NMR, 13 C NMR, HMQC, HMBC, 1H 1HCOSY and MS spectra. Combined with the literature data, it is confirmed that the structures are: 3 β-hydroxy-20 ethynostat acetate (1), 3 β-hydroxy 5,20 diepregnanone acetate (2) (3), 1,2 epoxy 3 ketones 2 0 en pregnane (4), 1,2 diene 3 ketones pregnane (5), 1 enone 3 ketone 2 0S methyl 2 1 hydroxyl (6), 1-enone ketone 2 0 β-hydroxyprogesterone acetate (7), 1-ene 3,2 2 diketocholesterol (8), 1,2,4-diene 3 Ketone 2 2, 2 3 dihydroxycholestane (9), O methylisogrifolin (10), (1R, 3S, 4S, 7E, 11E) 3α, 4α epoxy 7,1 1, Among them, compounds 4,6 and 10 were isolated from nature for the first time.For the first time, the spectral data of compounds 6, 7 and 10 Was reported and fully veri fi ed using 2D NMR. Compounds 1-6 and 7 showed inhibitory activity on human cervical cancer cells (HELA) with IC50 values of 3.3 and 6.3 mol / L, respectively. Compounds 7 and 7 inhibited hepatocellular carcinoma cells (BE740 2) And human leukemia cells (HL 60) also showed inhibitory activity with IC50 of 3.1 and 0.226 mol / L, respectively, and that of compound 11 on CO