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小麦种子从萌动至两叶期,用牛胸腺DNA分次进行滴浸处理。一代(D_1)株未出现明显变异,二代(D_2)株在蛋白含量、抗病性、株型、穗型、粒型和成熟期等方面均出现较大变异。在不同的年份间,用相同的缓冲液、技术,将牛DNA处理同一品种时,分年份处理的二代株中均出现性状相似的矮杆大穗变异类型,表现了该类型变异的重演性,其频率为1.95%～2.5%。经对D_1、D_2代变异株根尖和D_1代芽鞘细胞学观察,有染色体畸变存在。这表明牛胸腺DNA引入受体细胞后,可能在多方面作用于受体基因组,表现出复杂的综合效应。
Wheat seeds germinated to two-leaf stage, with bovine thymus DNA sub-drop dip treatment. The first generation (D_1) strain did not show obvious variation. The second generation (D_2) strain showed great variation in protein content, disease resistance, plant type, panicle type, grain type and maturity. In different years, when using the same buffer and technology to treat the same variety of bovine DNA, the type of short spike big panicle with similar traits appeared in the second-generation strains treated in different years, which showed the repeatability of this type of variation , Its frequency is 1.95% ~ 2.5%. Chromosome aberrations were found in the cell morphology of D_1 and D_2 progenies and D_1 generation buds. This suggests that the introduction of bovine thymus DNA into recipient cells may play a multifaceted role in the recipient’s genome and present a complex combination of effects.