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小麦离体芽鞘切段在稀释10倍的柠檬酸Na2HPO4缓冲液中的伸长量显著大于原液(原液由0.116mol/L的Na2HPO4+0.042mol/L的柠檬酸配成,pH5.6)中的。1.0~1.5cm芽鞘近基部4mm的切段在单位时间内的伸长量显著大于2.1~2.5cm芽鞘顶端3mm下的4mm切段,芽鞘越长,芽鞘切段伸长越慢。质量浓度≥5g/L的蔗糖对连籽粒芽鞘的伸长和根的生长有抑制作用,而对离体芽鞘伸长有显著的促进作用;20~40g/L蔗糖对芽鞘伸长的促进效果无显著差异。小麦感病品种矮秆早离体和连籽粒芽鞘切段受毒素的抑制率显著大于抗病品种望水白。
The elongation of wheat germplasm was significantly greater than that of the original solution in the citrate-Na2HPO4 buffer diluted 10 times (the stock solution was composed of 0.116 mol / L Na2HPO4 + 0.042 mol / L citric acid, pH 5.6) middle. The elongation of 4 mm cuttings of 1.0-1.5 cm bud sheath near the base was significantly larger than that of 4 mm cuttings of 3 mm at the top of 2.1-2.5 cm bud sheath in the unit time. The slower the segment extends. Sucrose with mass concentration≥5g / L could inhibit the elongation and root growth of the sheath of seedlings, but had a significant promoting effect on the elongation of sheath buds in vitro. The effects of 20 ~ 40g / L sucrose on the elongation of buds No significant difference in promotion effect. The susceptibilities of wheat susceptible cultivars Dwarf early-body and even seed-cuttings were significantly greater than that of the resistant varieties.