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目的:观察PINCH蛋白在口腔鳞状细胞癌中的表达情况,探讨其临床病理意义。方法:采用免疫组织化学ABC法,检测20例正常口腔粘膜和57例口腔鳞状细胞癌石蜡存档标本中PINCH蛋白的表达情况,分析其与患者临床病理特征的关系。结果:PINCH蛋白在正常口腔粘膜中少量表达,而在口腔鳞状细胞癌中的表达明显增加,尤其是在肿瘤的浸润边缘,二者比较有显著性差异(P=0.02);在口腔鳞状细胞癌中,淋巴结转移组阳性表达率为46.2%(12/26),淋巴结无转移组阳性表达率为29.0%(9/31),二者比较有显著性差异(P=0.02);PINCH蛋白的表达与患者性别、年龄、肿瘤大小、部位和分化程度无相关性(P>0.05)。结论:PINCH蛋白在口腔鳞状细胞癌的发生、发展中可能起着重要作用,并可作为一个预测肿瘤转移的新备选分子生物学指标。同时由于其表达在间质,为与肿瘤间质有关的抗肿瘤药物的开发提供了新思路和治疗靶点。
Objective: To observe the expression of PINCH protein in oral squamous cell carcinoma and to investigate the clinicopathological significance. Methods: Immunohistochemical ABC method was used to detect the expression of PINCH protein in 20 normal oral mucosa and 57 oral squamous cell carcinoma paraffin specimens. The relationship between PINCH protein expression and clinicopathological features was analyzed. Results: The PINCH protein was slightly expressed in normal oral mucosa and significantly increased in oral squamous cell carcinoma, especially at the margin of tumor invasion (P = 0.02). In oral squamous The positive rate of lymph node metastasis was 46.2% (12/26) in carcinoma and 29.0% (9/31) in lymph node without metastasis. There was significant difference between the two groups (P = 0.02). The positive rate of PINCH protein There was no correlation between the expression and the gender, age, tumor size, location and differentiation (P> 0.05). Conclusion: The PINCH protein may play an important role in the occurrence and development of oral squamous cell carcinoma and may be used as a new alternative molecular biomarker for predicting tumor metastasis. At the same time, because of its expression in the stroma, it provides new ideas and therapeutic targets for the development of anti-tumor drugs related to tumor stroma.