论文部分内容阅读
根据本实验室已获得的花生AhFUSCA3基因(NCBI登录号JX420284)序列,设计特异引物,构建原核表达载体,进行了重组蛋白表达分析,获得了分子量为42KD左右的目的蛋白条带;利用荧光定量PCR(Quantitative Real-time RT-PCR)方法,检测了AhFUSCA3基因在低温、高盐和干旱胁迫条件下的表达情况。结果显示,AhFUSCA3基因在低温和高盐处理的花生叶片中表达量明显上调,但在干旱处理的叶片中表达量则有明显下降。以上结果表明AhFUSCA3基因可能参与了花生对低温、高盐和干旱的抗性调控。
According to the sequence of AhFUSCA3 gene (NCBI accession number JX420284) obtained in this lab, specific primers were designed, prokaryotic expression vector was constructed, and the expression of recombinant protein was analyzed. The molecular weight of 42KD protein was obtained. Fluorescence quantitative PCR (Quantitative Real-time RT-PCR) method to detect AhFUSCA3 gene expression under low temperature, high salt and drought stress conditions. The results showed that the expression of AhFUSCA3 gene was significantly up-regulated in peanut leaves treated with low temperature and high salinity, but significantly decreased in leaves treated with drought stress. The above results indicated that AhFUSCA3 may be involved in the resistance of peanut to low temperature, high salinity and drought stress.