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目的探讨肠道病毒71型((Enterovirus 71,EV71)感染后脑、心、肺等重要器官组织病理学改变。方法选用1日龄BALB/c乳鼠30只,随机分为3组,每组10只。第1组为EV71模型组,第2组为正常对照组,第3组为灭活EV71病毒对照组,采用脑内接种途径,分别接种EV71毒液20μL/只、生理盐水20μL/只和灭活EV71毒液20μL/只。每日观察病症变化,濒临死亡动物做组织解剖及病理学检查,采用EV71VP1片段引物做RT-PCR进行病毒核酸检测。结果接种EV71动物在3d后出现后肢瘫痪,5～7d动物濒临死亡。组织病理学显示EV71感染鼠乳大脑皮质锥体细胞核出现红染现象,小脑颗粒层变薄,脑组织局部出血、液化。心肌纤维水肿变性、溶解、萎缩,正常结构消失,间质出血,少量炎细胞浸润。肺脏明显出血,肺泡隔轻度淤血,可见含铁血黄素颗粒。脊髓前角细胞核红染,少量淋巴细胞浸润。在大脑锥体细胞检测到EV71核酸片段。感染组乳鼠心肌及对照组乳鼠脑组织、心肌中未检测到EV71核酸片段。结论脑内接种EV71可致1日龄乳鼠感染,并可致感染乳鼠瘫痪及死亡。病理学检查损伤主要累及脑、心、肺、脊髓等,其发病表现及组织病理改变与人类EV71感染危重症有着很高的一致性。
Objective To investigate the histopathological changes of important organs such as brain, heart and lung in Enterovirus 71 (EV71) infection.Methods Thirty male BALB / c neonatal rats of 1 day old were randomly divided into three groups of 10 The first group was EV71 model group, the second group was the normal control group, the third group was inactivated EV71 virus control group, the intracerebral inoculation route was inoculated with EV71 venom 20μL / normal saline 20μL / Live EV71 venom 20μL / only.Different daily observation of disease changes, the verge of death of animal tissue anatomy and pathological examination, the use of EV71VP1 fragment primers for RT-PCR detection of viral nucleic acid results EV71 animals in the hind limb paralysis after 3d, 5 ~ 7d animals were dying.Histopathology showed red staining of pyramidal nuclei in EV71 infected mouse cerebellar cortex, thinning of cerebellar granular layer, local hemorrhage and liquefaction of brain tissue, degeneration of myocardial fiber edema, dissolution, atrophy and disappearance of normal structure Bleeding, a small amount of inflammatory cell infiltration .Long bleeding, mild alveolar congestion, visible hemosiderin particles .Anterior spinal cord nucleus stained red, a small amount of lymphocytic infiltration in the brain pyramidal cells detected EV71 nucleic acid tablets .The EV71 nucleic acid fragment was not detected in the myocardium and myocardium of neonatal and postnatal rats in the infected group.Conclusion Inoculation of EV71 can cause 1-day-old suckling mice to infect and cause paralysis and death in the neonatal rats.Pathological examination Injury mainly involving the brain, heart, lung, spinal cord, etc., its incidence and histopathological changes and human EV71 critically ill have a very high consistency.