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为探讨粒／巨噬细胞集落因子（GM－CSF）在继发性中耳胆脂瘤中的表达情况，分析其在胆脂瘤上皮增殖、骨质破坏特性中的作用，应用免疫组化SABC染色方法和计算机图像分析法，检测25例胆脂意上皮和10例正常外耳道皮肤中GM－CSF的表达情况。结果表明，GM－CSF在22例胆脂瘤上皮组织的全层细胞的胞浆中有强表达，阳性率为88％；GM－CSP在10例正常外耳道皮肤上皮中有弱表达，阳性率为40％。染色结果经计算机图像分析仪定量分析，两者的平均光密度分别为2．332和1．474，差异呈高度显著性（P＜0．05）。说明中耳胆脂瘤上皮中GM－CSF的含量显著高于正常外耳边皮肤的含量。推测GM－CSF可能参与了胆脂瘤的发生、发展及可能参与了胆脂病上皮增殖、骨质破坏的作用。
To investigate the expression of granulocyte / macrophage colony-stimulating factor (GM-CSF) in secondary middle ear cholesteatoma, the role of GM-CSF in the proliferation and destruction of cholesteatoma epithelium was analyzed. Immunohistochemical SABC Staining method and computer image analysis method to detect the expression of GM-CSF in 25 cases of cholecystolithiasis and 10 cases of normal external auditory canal skin. The results showed that GM-CSF was strongly expressed in the cytoplasm of the whole layer of 22 cholesteatoma epithelial tissues, with a positive rate of 88%. GM-CSF was weakly expressed in 10 cases of normal external auditory canal skin epithelium with a positive rate of 40%. The results of staining were analyzed quantitatively by computer image analyzer. The average optical density of the two was 2.332 and 1.474, respectively. The difference was highly significant (P <0.05). Description of middle ear cholesteatoma GM-CSF content was significantly higher than the normal outer ear skin content. It is speculated that GM-CSF may be involved in the occurrence and development of cholesteatoma and may be involved in the proliferation and destruction of cholesteatis epithelium.