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目的 研究内毒素血症时内毒素耐受大鼠肺部肿瘤坏死因子 -α(TNF α)和细胞因子诱导的中性粒细胞趋化物 (CINC)表达的情况。方法 SD大鼠随机分入正常对照组 (NC组 )和内毒素耐受组 (ET组 )。腹腔注射脂多糖 (LPS) ,每日 0 .6mg/kg连续 4d建立内毒素耐受模型。第 5天腹腔注射 6mg/kg。各组按注射大剂量LPS前 (0h) ,注射后 2、6、2 4、4 8、72h分为 6小组 ,n =6。用ELISA测定BALFTNF α。用RT PCR半定量检测肺组织CINCmRNA的表达。用SPSS 10 .0医学统计软件分析数据。结果 NC组注射LPS(6mg/kg)前 ,BALF中有少量TNF α ,肺组织有微量CINCmRNA表达 ;给药后两者均迅速上升 ,前者的高峰为 6h ,(10 .79±3.5 3) μg/L ,P =0 .0 0 1,后者的高峰出现于 2～ 6h(相对值为 1.6左右 ,P <0 .0 0 1) ,持续至 2 4h。而ET组两者均无上升。结论 内毒素耐受时 ,大剂量内毒素引起的肺部炎症反应和肺损伤减轻与肺部TNF α和CINC低表达有关
Objective To investigate the expression of tumor necrosis factor-α (TNFα) and cytokine-induced neutrophil chemoattractant (CINC) in the lung of endotoxin-tolerant rats during endotoxemia. Methods SD rats were randomly divided into normal control group (NC group) and endotoxin tolerance group (ET group). Peritoneal injection of lipopolysaccharide (LPS), 0.6 mg / kg daily for 4 consecutive days to establish endotoxin tolerance model. On the 5th day, intraperitoneal injection of 6mg / kg. Each group was injected with large dose of LPS (0h), 6,2,4,48,72h after injection were divided into 6 groups, n = 6. BALFTNF α was measured by ELISA. Semi-quantitative RT-PCR detection of CINC mRNA expression in lung tissue. Data was analyzed using SPSS 10 .0 medical statistics software. Results Before injection of LPS (6mg / kg) in NC group, there was a small amount of TNFα in BALF and slight expression of CINCmRNA in lung tissue. After administration, both of them increased rapidly, with the former peaked at 6h (10.79 ± 3.53) μg / L, P = .0 0 1, the latter peak appeared in 2 ~ 6h (relative value of about 1.6, P <0. 0 0 1), continued until 24h. There was no increase in both ET groups. Conclusions Endotoxin tolerance is related to the reduction of lung inflammation and lung injury induced by large doses of endotoxin and the low expressions of TNFα and CINC