Identification of superior clones by RAPD technology in Xanthoceras sorbifolia Bge.

来源 :Forestry Studies in China | 被引量 : 0次 | 上传用户:ok8000
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
It is very important to select and identify superior Xanthoceras sorbifolia Bge.trees for purposes of breeding and exploration.For our study,we selected superior X.sorbifolia trees using RAPD (random amplification of polymorphic DNA) technology.The results show that certain specific amplification bands emerged with significantly different frequencies between superior and non-superior trees.The 1400-bp band of No.2121 primer emerged in the superior trees at a frequency 2.4 times that of the non-superior trees and the 600-bp band of No.2153 primer in the non-superior trees at a frequency 2.5 times that of the superior trees.There were very significant differences between the superior and non-superior trees.The frequency of the 1600-bp band of No.1161 primer was the same in both kinds of trees,but significant differences were observed in point frequency (the frequency of the superior trees is 1.4 times that of the non-superior trees).These bands can be used as important targets to distinguish between superior and non-superior trees.The use of a combination of amplified bands simultaneously can play a guiding role in the selection of superior X.sorbifolia trees. It is very important to select and identify superior Xanthoceras sorbifolia Bge. Trees for purposes of breeding and exploration. For our study, we selected superior X. sorbifolia trees using RAPD (random amplification of polymorphic DNA) technology. Results show that certain specific amplification bands emerged with significantly different frequencies between superior and non-superior trees. The 1400-bp band of No. 2112 primer emerged in the superior trees at a frequency 2.4 times that of the non-superior trees and the 600-bp band of No. 2153 primer in the non-superior trees at a frequency 2.5 times that of the superior trees. There was very significant differences between the superior and non-superior trees. The frequency of the 1600-bp band of No.1161 primer was the same in both kinds of trees, but significant differences were observed in point frequency (the frequency of the superior trees is 1.4 times that of the non-superior trees) .sese bands can be used as important targets to disting uish between superior and non-superior trees. The use of a combination of amplified bands simultaneously can play a guiding role in the selection of superior X. sorbifolia trees.
其他文献
弘一法师有言:“问余何适,廓尔妄言;华枝春满,天心月圆。”大意是说春满花开,皓月当空的地方就是他梦想的归宿。阳光熹微,留下温暖;风过有痕,吹弄华章。这是他们最光荣的使命与梦想,折射出的是弘一法师那属于国人的对信仰永不停息的追求。  庄子是这样的人。“宁曳尾于途中”,不肯外出做官,他在梦中与蝴蝶、鲲鹏嬉戏,腰系一个葫芦便去浪迹天涯。他之后,无数古代文人随他而去:那个不为五斗米折腰的陶渊明去了,种豆南
期刊
这一季雨,是出梅之后下起来的,之前它好像没有睡醒.六七月的天,摸不透脾性,原本是除了热,除了蝉鸣、蛙声的聒噪,什么都没有的,却来了几场无休无止的大雨.雨帘把慵懒的大地惹
期刊
上个世纪,在数宁技术的推动和影响下,计算机图形学诞生了.而且其研究范围也越来越广.经过对比,不难发现,计算机图形学所研究的内容尽然与平面设计元素及术语如此类似,像是为
一首诗顺读流畅优美,若将它的全篇或句、段倒回来读,也流畅优美,这就是回文诗.这种诗虽带有一定的文字游戏性质,但它构思巧妙,手法独特,音韵和谐,趣味盎然,是我国诗苑中的一
期刊
一只蚂蚁爬到海岸边,望着一个接一个的海浪涌到岸上,不禁忧愁起来:“海这么大,而我这么小,我一辈子也不能看见大海的尽头……我还活在世上干什么呢?”rn蚂蚁在一棵棕榈树下坐
期刊
相隔近三十年,去年国庆节,终于再次重游郴州苏仙岭.在迷蒙的晨雾中,拾阶而上,竹林幽径,绿草苍苔,晕染出一片凄清.rn山崖上的三绝碑已在小院深锁,不得而见,我只能望门兴叹,寂
期刊
大局意识与热点引导□肖获一个记者,孜孜以求的就是要写出有分量、有深度、有新意、有文采的好稿来;一个中央大报驻省市记者,更要写出既有地方特色、更有广泛指导意义的好稿来。
在解决有关函数问题时,容易出现错误,现举例加以分析,希望你能从中吸取教训,避免犯类似的错误.rn一、没有掌握对称点的坐标特征rn例1 在平面直角坐标系中,点A与点B关于y轴对
期刊
炎炎夏日,酷暑难耐.古人或栖于树阴下,或倚靠在水畔亭旁,避暑的方式完全是自然的.于是,便有了许多脍炙人口的纳凉诗.闲暇之时,品味这些诗作,既能感受到诗人的避暑之乐,又能走
期刊
函数是初中数学的重要内容.我们主要学习了一次函数、反比例函数和二次函数,它们在生活中的应用非常广泛,请看下面的例子.rn一、生活中的函数模型rn例1 如图1,一种斜挎包的挎
期刊