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为了探索大苞短毛唇柱苣苔离体培养和快速繁殖的最佳培养条件。以大苞短毛唇柱苣苔叶片为外植体,建立其组培快繁再生体系。结果表明大苞短毛唇柱苣苔叶片外植体的最优消毒方法为:采用75%酒精灭菌15 s,0.1%Hg Cl2灭菌4 min;叶片诱导不定芽最佳培养基为:MS+TDZ0.5 mg/L+NAA0.1 mg/L,诱导率为83.75%;不定芽增殖最佳培养基为MS+6-BA0.5 mg/L+NAA0.1 mg/L,增殖系数可达16,且不定芽生长良好;生根培养基以1/2MS+IBA0.1 mg/L为最好,生根率达96.67%以上。组培苗经炼苗及移栽,成活率达95%以上。建立了大苞短毛唇柱叶片组织培养的快繁体系,为规模化生产大苞短毛唇柱苣苔提供技术指导。
In order to explore the best cultivation conditions in vitro and rapid propagation of Bupleurum chinense. In order to explant the rapid growth and reproduction system of tissue culture, The results showed that the best method of disinfection of explants was: sterilizing with 75% alcohol for 15 s and sterilizing with 0.1% Hg Cl2 for 4 min. The best medium for inducing adventitious buds was MS + TDZ0.5 mg / L + NAA0.1 mg / L, the induction rate was83.75%. The best proliferation medium for adventitious buds was MS + 6-BA0.5 mg / L + NAA0.1 mg / L, Reached 16, and adventitious buds grew well. The rooting medium was best with 1 / 2MS + IBA0.1 mg / L and the rooting rate reached 96.67%. Tissue culture seedlings by transplanting and transplanting, survival rate of 95% or more. A rapid propagating system was established for the tissue culture of Dabaobei lipsticks, which provided technical guidance for the large-scale production of Daphnia magna.