论文部分内容阅读
目的:建立扶芳藤药材中原儿茶酸的含量测定方法。方法:利用HPLC测定扶芳藤中原儿茶酸的含量,采用Ulitimate XB-C18(2)色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.6%冰醋酸水溶液(27∶73),柱温室温,流速0.7 mL·min-1,检测波长260 nm,进样量10μL。结果:原儿茶酸的回归方程为A=1.395×107C-1.12×104(r=0.999 9)。原儿茶酸在0.005~1μg呈良好的线性关系。原儿茶酸的平均回收率为105.3%,RSD 1.52%。结论:采用此法测定扶芳藤中原儿茶酸的含量,准确可靠,可用于该药材的质量控制。
Objective: To establish a method for the determination of Zhongyuan catechin in Euonymus fortunei. Methods: The content of protocatechuic acid in Radix Euphraticae was determined by HPLC. Ulitimate XB-C18 (2) column (4.6 mm × 250 mm, 5 μm), mobile phase methanol-0.6% Column temperature room temperature, flow rate 0.7 mL · min-1, detection wavelength 260 nm, injection volume 10μL. Results: The regression equation of protocatechuic acid was A = 1.395 × 107C-1.12 × 104 (r = 0.999 9). Protocatechuic acid in 0.005 ~ 1μg showed a good linear relationship. The average recovery of protocatechuic acid was 105.3%, RSD 1.52%. Conclusion: This method is used to determine the content of protocatechuic acid in Euonymus fortunei, which is accurate and reliable and can be used for the quality control of this herb.