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目的探讨胃癌组织中Akt2、HER2蛋白表达情况及HER2蛋白与胃癌临床病理学特征的关系。方法将90例原发性胃癌患者作为研究对象,同时选取同期行胃镜检查的90例健康体检者作为对照。两组患者均采用免疫组织化学SP法检测胃粘膜组织中的Akt2、HER2蛋白表达情况。比较两组Akt2、HER2蛋白的阳性表达情况,并分析胃癌分化程度、浸润深度、肿瘤直径、肿瘤位置、淋巴结转移及TNM分期等病理学指标与HER2的关系。结果胃癌组Akt2、HER2蛋白阳性表达分别为51例(56.67%)、19例(21.11%),与健康组相比差异均具有统计学意义(P<0.05)。胃癌患者中,Akt2、HER2均为阳性表达的患者有17例,均为阴性表达的患者有37例,Akt2表达阳性而HER2阴性的患者有34例,HER2表达阳性而Akt2阴性的患者有2例。Spearman相关分析发现,在胃癌组织中HER2与Akt2表达具有相关性(γ=0.342,P=0.001<0.05)。HER2表达与胃癌分化程度、浸润深度、肿瘤位置、淋巴结转移及TNM分期显著相关(P<0.05);而与肿瘤直径无相关性(P>0.05)。结论胃癌组织中Akt2、HER2蛋白表达水平增高,且HER2表达与病理学指标相关,能在一定程度上反映胃癌患者的预后情况。
Objective To investigate the expression of Akt2 and HER2 in gastric cancer and the relationship between HER2 protein and clinicopathological features of gastric cancer. Methods Ninety patients with primary gastric cancer were enrolled in this study. 90 healthy subjects undergoing gastroscopy during the same period were selected as controls. Immunohistochemical SP method was used to detect the expression of Akt2 and HER2 in gastric mucosa in both groups. The positive expression of Akt2 and HER2 protein in the two groups were compared. The relationship between the expression of HER2 and the degree of differentiation, depth of invasion, tumor diameter, tumor location, lymph node metastasis and TNM stage were analyzed. Results The positive expressions of Akt2 and HER2 in gastric cancer group were 51 (56.67%) and 19 (21.11%) respectively, which were significantly different from those in healthy group (P <0.05). Among the gastric cancer patients, 17 were positive for both Akt2 and HER2, 37 were negative, Akt2 was positive and HER2 negative were 34, HER2 was positive and Akt2 was negative . Spearman correlation analysis showed that HER2 was correlated with Akt2 expression in gastric cancer tissues (γ = 0.342, P = 0.001 <0.05). The expression of HER2 was significantly correlated with the degree of differentiation, depth of invasion, tumor location, lymph node metastasis and TNM stage (P <0.05), but not with tumor diameter (P> 0.05). Conclusion The expression of Akt2 and HER2 in gastric cancer tissues is increased, and the expression of HER2 is correlated with pathological parameters, which can reflect the prognosis of gastric cancer to a certain extent.