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目的探讨兔蛛网膜下腔出血(SAH)后血清和脑脊液(CSF)中S100B蛋白的变化及其意义。方法采用枕大池二次注血法制作SAH模型,动物随机分为正常组、穿刺组、盐水对照组和SAH组,正常组于饲养观察3d后取其血清及CSF,其余各组分别于建模后1 h、3 d、5 d、7 d、10 d取血清及脑脊液。应用双抗体夹心ELISA法检测各组血清及CSF中S100B蛋白的浓度。数据结果应用统计软件SPSS13.0进行处理。结果SAH组血清及CSF中S100B蛋白浓度在各个时间点均明显高于其余3组(P=0),并呈现血清S100B蛋白浓度于SAH后1 h即开始升高,3～5 d达到高峰后逐渐恢复,而CSF中S100B则于SAH后1 h升高后稍下降;再于5～7 d第二次达到高峰的变化。盐水组则呈现血清和CSF中S100B蛋白于造模后1 h高于穿刺组及正常组(P<0.05),然后迅速下降至正常。结论SAH后血清与CSF中S100B蛋白浓度呈明显的动态变化。测定血清与CSF中S100B蛋白浓度对判断SAH后血脑屏障(BBB)的病理生理改变具有重要意义。
Objective To investigate the changes of S100B protein in serum and cerebrospinal fluid (CSF) after subarachnoid hemorrhage (SAH) in rabbits and its significance. Methods The SAH model was made by the secondary injection of occipital cistern. The animals were randomly divided into normal group, puncture group, saline control group and SAH group. The normal group was fed with serum and CSF for 3 days, and the other groups were divided into model After 1 h, 3 d, 5 d, 7 d, 10 d serum and cerebrospinal fluid were collected. Double antibody sandwich ELISA was used to detect the concentration of S100B protein in serum and CSF. The results of the application of statistical software SPSS13.0 for processing. Results Serum and CSF levels of S100B protein in SAH group were significantly higher than those in the other three groups at each time point (P = 0). Serum levels of S100B protein increased at 1 h after SAH, and reached peak at 3 to 5 d Gradually recovered. However, S100B in CSF was slightly decreased at 1 h after SAH, and peaked at 5 ~ 7 d for the second time. In saline group, S100B protein in serum and CSF was higher at 1 hour after modeling than that in puncture group and normal group (P <0.05), and then rapidly decreased to normal. Conclusion The concentration of S100B in serum and CSF after SAH showed a significant dynamic change. Determining the concentration of S100B protein in serum and CSF is of great importance in determining the pathophysiological changes of the blood-brain barrier (BBB) after SAH.