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运用有性杂交方法,以强辣灌木辣椒Capsicum frutescens H101为母本(P1)、强辣C.chinense PI439487为父本(P2)进行种间杂交,获得了C.frutescens×C.chinense种间杂种。对种间杂种F125个表型性状进行了观察比较,结果表明:F1在生长势方面具有明显的杂种优势,其他表型性状则多介于P1与P2之间。SRAP分析显示,F1与P1、P2共有带220条,占总位点数的53.92%,与P1或P2共有带143条,占35.05%,F1特异带和特异缺失带12条,点2.95%。F1与P1遗传相似系数为0.749,大于其与P2的遗传相似系数(0.740),说明杂种在DNA水平上更趋向于母本。C.frutescens×C.chinense种间杂种的获得,为C.chinense香味和多花基因的转移及新材料创制奠定了基础。
The cross of C.frutescens × C.chinense interspecific hybrids was obtained by sex hybridization method with the cross of Capsicum frutescens H101 as the female parent (P1) and C. chinense PI439487 as the male parent (P2) . F125 phenotypic traits of interspecific hybrids were observed and compared. The results showed that: F1 had obvious heterosis in growth potential, while other phenotypic traits were mostly between P1 and P2. SRAP analysis showed that F1 shared a total of 220 bands with P1 and P2, accounting for 53.92% of total loci. There were 143 bands with P1 or P2, accounting for 35.05%. There were 12 F1 and 12.95 specific bands. The genetic similarity coefficient between F1 and P1 was 0.749, which was greater than the genetic similarity coefficient between P2 and F1 (0.740), indicating that the hybrids tended to be more female at the DNA level. C.frutescens × C.chinense Interspecific hybrids provide the foundation for C.chinense scent and multi-gene transfer and creation of new materials.