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目的探讨子宫内膜癌组织中雌激素受体(ER)亚型mRNA及孕激素受体(PR)亚型mRNA和蛋白表达水平的变化及其意义。方法采用RT-PCR法检测66例子宫内膜癌和30例正常子宫内膜组织ER、PR亚型mRNA的表达,采用蛋白印迹法检测PR亚型蛋白的表达。结果(1)ERαmRNA在子宫内膜癌和正常子宫内膜组织中的表达水平分别是8.00±7.77、3.84±2.57,而ERβmRNA的表达水平分别是4.15±3.55、0.41±0.29,子宫内膜癌组织中ERα、ERβmRNA表达水平均高于正常子宫内膜,两种组织间分别比较,差异均有统计学意义(P<0.05)。(2)PR、PRA和PRB蛋白表达及PR mRNA表达的降低与子宫内膜癌的发生有关(P<0.05),而PRA与PRB蛋白表达的比值和PRB mRNA的表达与子宫内膜癌的发生无明显相关性(P值分别为0.550、0.901)。(3)子宫内膜癌组织中PRB mRNA与ERβmRNA的表达水平呈正相关关系(r=0.43,P<0.01)。结论子宫内膜癌组织中ER亚型mRNA表达上调、PR蛋白和mRNA表达的下调可能参与了子宫内膜癌的发生;PRB mRNA与ERβmRNA表达呈正相关关系。
Objective To investigate the changes of estrogen receptor (ER) isoform and progesterone receptor (PR) isoform mRNA and protein expression in endometrial carcinoma and its significance. Methods The mRNA expression of ER and PR in 66 cases of endometrial carcinoma and 30 cases of normal endometrium was detected by RT-PCR. The expression of PR subtype was detected by Western blotting. Results (1) The expression levels of ERα mRNA in endometrial carcinoma and normal endometrium were 8.00 ± 7.77 and 3.84 ± 2.57, respectively, while the expression levels of ERβ mRNA were 4.15 ± 3.55 and 0.41 ± 0.29, respectively The expression levels of ERα and ERβmRNA in normal endometrium were higher than those in normal endometrium, the difference was statistically significant (P <0.05). (2) The expression of PR, PRA and PRB and the decrease of PR mRNA expression were correlated with the occurrence of endometrial carcinoma (P <0.05), while the ratio of PRA and PRB protein expression and PRB mRNA expression were correlated with the occurrence of endometrial carcinoma No significant correlation (P values were 0.550, 0.901). (3) The expression of PRB mRNA and ERβmRNA in endometrial carcinoma was positively correlated (r = 0.43, P <0.01). Conclusions The ER subtypes mRNA expression is upregulated in endometrial carcinoma, and the downregulation of PR protein and mRNA may be involved in the occurrence of endometrial carcinoma. PRB mRNA and ERβ mRNA expression are positively correlated.