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本文介绍一种感染曼氏血吸虫尾蚴固定小鼠的装置。这种装置简便,价钱便宜而实用。这种装置是一块长72cm、宽7cm、厚0.5cm的光滑木板。板上有5个直径为0.5cm、间隔12cm的孔眼。每个中心孔眼的侧面,在板的边缘钉着两对等距离的1cm长的不锈钢图钉。尾部感染的步骤:将小鼠的尾巴穿过选定的孔眼,用棉线把小鼠的脚爪扎紧在侧面的图钉上使小鼠固定作仰卧姿势。将一排盛有尾蚴水的烧杯放在板下作为本装置的支座,感染过程与其他尾浸法相似。腹部的小圈法感染步骤:轻轻剃去小鼠的腹面毛,清洗并擦干皮肤,将定量的尾蚴水慢慢滴在固定在皮肤上的塑料小圈内。小鼠脚未出现因固定发生坏死现象,而且用此种
This article describes a device that infects cercariae of Schistosoma mansoni to immobilize mice. This device is simple, cheap and practical. This device is a smooth 72cm long, 7cm wide, 0.5cm thick smooth wood. There are five holes on the board that are 0.5 cm in diameter and 12 cm apart. On the side of each center eyelet, two pairs of equally spaced 1-cm-long stainless steel pins are nailed to the edge of the plate. Tail infection steps: Mouse tail through the selected hole, with a cotton thread to the mouse paw claws on the side of the pin to make the mouse fixed supine position. Place a row of beakers containing cercariae water on the bottom of the plate as a standoff for this device. The infection process is similar to other tailspans. Abdomen small circle infection steps: gently shave the ventral hair of mice, clean and dry the skin, the quantitative cercariae water slowly drip fixed in the plastic ring on the skin. Mouse feet did not appear due to fixed occurrence of necrosis, but also with this