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仿刺参(Apostichopus japonicus)体质量(0.65±0.03)g,基础饲料中添加5%(质量分数)全营养破壁酵母为实验饲料,以基础饲料为对照。分别于投喂后第15天和第30天检测仿刺参体腔细胞数量、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)活性,于第30天计数肠道菌群。结果显示,实验组仿刺参体腔细胞数量与对照组差异不显著;ACP、AKP、CAT活性均比对照组有所增高,其中第15天CAT活性和第30天AKP活性与对照组具有显著性差异(P<0.05)。投喂破壁酵母对仿刺参肠道菌群有一定影响,可显著降低肠道内异养菌总数及弧菌数量。研究表明,全营养破壁酵母对仿刺参非特异性免疫活性有增强作用,且对肠道有害菌群有一定抑制作用,但其对肠道菌群的影响及适宜添加量等需进一步研究。本研究旨在探索全营养破壁酵母在海参育保苗中的作用机理,以期为仿刺参安全健康养殖开发新型绿色添加剂提供科学依据。
The body weight (Apostichopus japonicus) of Apostichopus japonicus (0.65 ± 0.03) g, basal diet supplemented with 5% (mass fraction) whole-nutrition broken-wall yeast was the experimental feed, and the basal diet was the control. At the 15th and the 30th day after feeding, the number of cells, acid phosphatase (ACP), alkaline phosphatase (AKP) and catalase (CAT) Road flora. The results showed that there was no significant difference between the experimental group and the control group in the number of cells in the acanthopanax senticosus group. The activities of ACP, AKP and CAT were higher than those in the control group. CAT activity on the 15th day and AKP activity on the 30th day were significantly higher than those in the control group Difference (P <0.05). Sowing broken yeast on the ginseng intestinal flora have a certain impact, can significantly reduce the total number of intestinal bacteria and Vibrio bacteria. Studies have shown that the full-nutrition broken yeast enhances the nonspecific immune activity of the sea cucumber, but also inhibits the harmful intestinal flora, but its effect on the intestinal flora and the appropriate dosage need further study. The purpose of this study was to explore the mechanism of full-nutrition broken yeast in sea cucumber seedling growth and seedling, in order to provide a scientific basis for the development of new green additives for the safe and healthy breeding of sea cucumber.