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目的利用纳米金标基因芯片检测前列腺癌(prostatic cancer,PCa)特异性多基因,探讨其临床意义。方法应用纳米金标基因芯片对11个PCa特异性基因靶标(IGF1、P27、AR、PSMA、KLK3、PCNA、Ki-67、KLK1、KLK2、TMPRSS2、SREBF2)进行检测,对各基因表达进行研究。结果纳米金标基因芯片检测单链核酸的灵敏度达到80f mol/L,在优化条件下,目标上调基因呈阳性表达,下调及阴性对照无显色,可肉眼分辨。结论纳米金标基因芯片技术检测PCa特异性基因方法快速简单,灵敏度高,特异性好,可对选定的11个目标基因定性及半定量检测,不需要借助于特殊仪器,操作简单,样本保存时间长,在普通实验室即可完成。纳米金标基因芯片检测有望成为PCa早期诊断的新一代芯片检测系统。
Objective To detect prostatic cancer (PCa) -specific multi-gene using nano gold standard gene chip and to explore its clinical significance. Methods Eleven PCa-specific gene targets (IGF1, P27, AR, PSMA, KLK3, PCNA, Ki-67, KLK1, KLK2, TMPRSS2 and SREBF2) were detected by gold- Results The sensitivity of single-stranded nucleic acid was up to 80f mol / L with gold-labeled DNA microarray. Under optimized conditions, the target up-regulated gene was positive, while down-regulated and negative control showed no color and could be visually identified. Conclusion The method of gold nanoparticle gene chip for detecting PCa-specific genes is rapid, simple, sensitive and specific. It can detect 11 selected target genes qualitatively and semi-quantitatively without the need of special instruments. The method is easy to operate and the sample is saved A long time, in the normal laboratory can be completed. Nanometer gold standard gene chip detection is expected to become a new generation of PCa early detection chip detection system.