鼠抗人CD28功能性单克隆抗体的研制及其生物学特性的鉴定

• 作 者: 居颂文 刘琳 居颂光 於葛华 潘建忠 邱玉华 张学光
• 机 构: 苏州大学生物技术研究所,苏州大学生物技术研究所,苏州大学生物技术研究所,苏州大学生物技术研究所,苏州大学生物技术研究所,苏州大学生物技术研究所,苏州大学生物技术研究所 苏州 215007,苏州 215
• 出 处: 上海免疫学杂志
• 发表日期: 2003年05期
• 关键词: CD28 杂交瘤 多发性骨髓瘤 单克隆抗体

以天然表达CD28分子的人多发性骨髓瘤(multiple myeloma,MM)细胞:XG-1为免疫原,常规免疫BALB/c小鼠,采用B淋巴细胞杂交瘤技术进行细胞融合,CD28转基因细胞株(T-28)及XG-1为抗体筛选阳性细胞株,经免疫荧光标记分析反复筛选和多次的克隆化培养,最终获得1株持续、稳定分泌鼠抗人CD28单克隆抗体的杂交瘤细胞株,命名为10F3。对这株单抗生物学功能的研究结果表明,这株抗体能特异性地识别人CD28分子和介导有效的共刺激信号,激发T细胞的活化和增殖。

Human multiple myeloma (MM) cells, which naturally express CD28 molecules, were immunized with XG-1 and BALB / c mice were routinely immunized with the B lymphocyte hybridoma technique. CD28 transgenic T-28) and XG-1 were screened by antibody. After repeated screening by immunofluorescence and repeated cloning and culture, a hybridoma cell strain that consistently and stably secreted mouse anti-human CD28 monoclonal antibody was obtained , Named 10F3. The biological function of this monoclonal antibody showed that this antibody specifically recognizes human CD28 molecules and mediates potent co-stimulatory signals and stimulates T cell activation and proliferation.