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目的探讨胎盘特异性基因4(PLAC4)mRNA在不同孕期孕妇血浆中表达的动态变化。方法经羊水穿刺核型分析确诊为孕正常整倍体胎儿的单胎妊娠孕妇82例(对照组),按收集血样时的不同孕周范围(17-23周)进行分组,采用定量PCR法检测PLAC4mRNA的表达水平。同时对18例孕非整倍体胎儿孕妇血浆(病例组,其羊水核型分析均为21三体胎儿)PLAC4mRNA行定量分析。比较PLAC4mRNA在不同孕期及两组间的含量差异。结果对照组血浆中PLAC4mRNA在17-18周(中孕)时的表达水平低于20-23孕周(P<0.05);对照组与病例组间的胎儿PLAC4mRNA含量无统计学差异(P>0.05)。结论中孕期孕妇血浆中胎儿游离PLAC4mRNA含量在20-23孕周时较高;中孕期21三体孕妇血浆中胎儿基因PLAC4mRNA含量与孕正常整倍体胎儿的孕妇无统计学差异。
Objective To investigate the dynamic changes of placenta-specific gene 4 (PLAC4) mRNA expression in pregnant women at different stages of pregnancy. Methods 82 cases of singleton pregnancy pregnant women with normal ectopic pregnancy confirmed by amniocentesis and karyotype analysis were divided into different gestational weeks (17-23 weeks) at the time of collecting blood samples and then detected by quantitative PCR PLAC4 mRNA expression levels. At the same time, quantitative analysis of PLAC4 mRNA in 18 pregnant aneuploid fetuses’ pregnant women’s plasma (cases group, their amniotic karyotype analysis was 21 trisomy 21). PLAC4 mRNA was compared between different groups during pregnancy and between groups. Results The plasma level of PLAC4 mRNA in control group was lower than that in 20-23 gestational weeks (P <0.05) at 17-18 weeks (P <0.05). There was no significant difference in PLAC4 mRNA in control group and case group (P> 0.05 ). Conclusions The content of fetal PLGA4 mRNA in pregnant women during the second trimester is higher at 20-23 gestational weeks. There is no significant difference in the PLAC4 mRNA level in the second trimester pregnant women between the first trimester and the second trimester.