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利用RT-PCR手段克隆得到1个毛白杨CBF基因cDNA,命名为PtCBF5(GenBank注册:DQ354395)。PtCBF5全长837bp,该序列包括起始密码子ATG和42bp的5′末端非编码区,终止密码子TGA和98bp的3′末端非编码区,开放阅读框编码231个氨基酸。RT-PCR半定量研究表明:PtCBF5在叶片组织中的表达量高,茎、根中的表达量低。组培苗在低温、干旱、NaCl、ABA条件下处理诱导24h,在低温和干旱的条件下PtCBF5表达量在诱导30min后开始增加,分别在2h和6h达到峰值,24h后又恢复到初始水平;而高盐和ABA存在的条件下未有显著变化。PtCBF5在植物体适应寒冷和干旱的过程中可能有着重要作用,尤其在逆境初期。
One Populus tomentosa CBF cDNA was cloned by RT-PCR and named as PtCBF5 (GenBank registration: DQ354395). The full length of PtCBF5 was 837bp. The sequence included the start codon ATG and 42bp 5 ’non-coding region, stop codon TGA and 98bp 3’ non-coding region. The open reading frame encoded 231 amino acids. RT-PCR semi-quantitative studies showed that: PtCBF5 expression in leaf tissue is high, low expression in stems and roots. Under the conditions of low temperature and drought, the expression of PtCBF5 began to increase 30min after induction, reached its peak at 2h and 6h respectively and returned to the initial level after 24h. No significant changes were observed in the presence of high salt and ABA. PtCBF5 may play an important role in the adaptation of plants to cold and drought, especially in the early adversity.