【摘 要】
：
目的研究灵芝多糖对肺癌患者血清淋巴细胞活化抑制的作用.方法将含有终浓度为0、0.2、0.8、3.2、12.8 mg/L 灵芝多糖的肺癌患者血清与肺癌患者的淋巴细胞进行体外培养,以健康
【机 构】
：
河北省沧州荣复军人疗养院,承德医学院附属医院
论文部分内容阅读
目的研究灵芝多糖对肺癌患者血清淋巴细胞活化抑制的作用.方法将含有终浓度为0、0.2、0.8、3.2、12.8 mg/L 灵芝多糖的肺癌患者血清与肺癌患者的淋巴细胞进行体外培养,以健康人血清代替肺癌患者血清为对照.采用四甲基偶氮唑盐(MTT)比色法检测淋巴细胞的增殖活性,用流式细胞仪检测淋巴细胞 CD69的表达.结果在肺癌患者血清作用下,植物血凝素(PHA)诱导的淋巴细胞增殖活性〔吸光度(A)值〕和 CD69阳性表达率以未加灵芝多糖的肺癌血清组最低,健康对照组最高.与健康对照组比较,未加灵芝多糖的肺癌血清组淋巴细胞增殖活性(0.654±0.029比1.213±0.106)、CD69阳性率〔(33.04±16.76)%比(74.18±12.16)%〕均明显降低(均 P
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