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目的:进行菘蓝四倍体株系形态学、细胞学及流式细胞术鉴定。方法:将进行了根尖压片染色体计数鉴定的菘蓝四倍体株系组培苗,经生根、炼苗后移栽大田,以田间栽培苗为实验材料。宏观观察进行外观形态鉴定;徒手切片法进行观察,研究叶片的气孔长度、宽度、气孔密度;用流式细胞仪法进行倍性鉴定。结果:四倍体植株和二倍体植株相比,外观形态发生明显的变化;四倍体株系的气孔长度均显著大于二倍体,气孔保卫细胞叶绿体数目均显著多于二倍体植株;流式细胞术鉴定证明供试材料均为四倍体。结论:供试材料是四倍体,而且宏观观察、微观气孔长度、保卫细胞叶绿体数目可进行诱变材料倍性的辅助鉴定,同时流式细胞术可用于菘蓝的倍性鉴定。
OBJECTIVE: To determine the morphology, cytology and flow cytometry of tetraploid plants. Methods: Tissue culture seedlings of tetraploid blue tetraploid strain with chromosome count of apical plaque were planted and transplanted into the field after being rooted and hardened. Field cultivated seedlings were used as experimental materials. Macroscopic observation of the appearance of morphological identification; free-hand slicing method to observe the stomatal length, width, stomatal density; flow cytometry ploidy identification. Results: The morphology of tetraploid plants changed obviously compared with that of diploid plants. The stomatal length of tetraploid plants was significantly higher than that of diploid plants, and the number of stomatal guard cells was significantly more than that of diploid plants. Flow cytometry identification test materials are tetraploid. CONCLUSION: The tested material is tetraploid, and the macroscopic observation, the microscopic stoma length and the number of chloroplasts in guard cells can be used to identify the ploidy of the mutagenized material, and the flow cytometry can be used to identify the ploidy of Cyanobacteriaceae.