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目的:探讨子宫内膜癌组织中热休克转录因子1(heat-shock transcription factor 1 HSF1)在子宫内膜癌组织中的的表达及临床意义。方法:应用免疫组织化学法检测17例正常子宫内膜、28例不典型增生及101例子宫内膜癌组织中HSF1和雌激素受体α、β(ERα、β)的表达情况,观察在子宫内膜组织中HSF1表达情况。分析其与年龄、临床分期、组织学分级、浸润程度、淋巴结转移间以及雌激素表达的关系。结果:HSF1在正常子宫内膜中的阳性表达率为52.9%,在不典型增生组织中的阳性表达率67.9%,在子宫内膜癌中的阳性表达率为77.2%,HSF1蛋白表达强度在正常、不典型增生和内膜癌组间比较有统计学差异(P<0.05)。HSF1表达与病理分型、肌层侵润深度、淋巴结转移以及雌激素表达有关(P<0.05),与年龄、临床分期无关。ERα正常子宫内膜中的阳性表达率为88.2%,在不典型增生组织中的阳性表达率85.7%,在子宫内膜癌中的阳性表达率为55.4%,ERα表达强度在正常、不典型增生和内膜癌组间比较有统计学差异(P<0.05)。ERβ正常子宫内膜中的阳性表达率为76.5%,在不典型增生组织中的阳性表达率71.4%,在子宫内膜癌中的阳性表达率为51.0%,ERβ表达强度在正常、不典型增生和内膜癌组间比较有统计学差异(P<0.05)。结论:HSF1在正常子宫内膜、子宫内膜不典型增生、子宫内膜癌中的表达逐渐增高并表现为促癌作用,ERα、β在正常子宫内膜、子宫内膜不典型增生、子宫内膜癌中的表达逐渐增高并表现为抑癌作用,在ERα、β阴性的癌组织中HSF1这种促癌作用更明显,这有助于为子宫内膜癌患者制定个体化治疗方案以及进一步研究诊治中提供依据。
Objective: To investigate the expression of heat-shock transcription factor 1 (HSF1) in endometrial carcinoma and its clinical significance. Methods: Immunohistochemistry was used to detect the expression of HSF1 and estrogen receptor alpha, beta (ER alpha, beta) in 17 cases of normal endometrium, 28 cases of atypical hyperplasia and 101 cases of endometrial carcinoma. Endometrial HSF1 expression. The relationship with age, clinical stage, histological grade, degree of infiltration, lymph node metastasis and estrogen expression was analyzed. Results: The positive expression rate of HSF1 in normal endometrium was 52.9%, in atypical hyperplasia tissue was 67.9%, in endometrial carcinoma was 77.2%, and that of HSF1 protein in normal , Atypical hyperplasia and endometrial cancer were statistically significant differences (P <0.05). The expression of HSF1 was correlated with pathological type, depth of myometrial invasion, lymph node metastasis and estrogen expression (P <0.05), but not with age and clinical stage. The positive expression rate of ERα in normal endometrium was 88.2%, the positive expression rate in atypical hyperplasia tissue was 85.7%, the positive expression rate in endometrial carcinoma was 55.4%. The expression level of ERα in normal and atypical hyperplasia And endometrial cancer were statistically significant difference (P <0.05). The positive expression rate of ERβ was 76.5% in normal endometrium, 71.4% in atypical hyperplasia tissues, and 51.0% in endometrial carcinoma. The expression of ERβ in normal endometrial dysplasia And endometrial cancer were statistically significant difference (P <0.05). Conclusion: The expression of HSF1 in normal endometrium, endometrial dysplasia and endometrial carcinoma gradually increases and shows the function of promoting cancer. The expressions of ERα and β in normal endometrium, endometrial atypical hyperplasia, intrauterine The expression of membrane cancer gradually increased and showed a tumor suppressor role in the ERα, β-negative cancerous tissue HSF1 such a more obvious role in promoting cancer, which helps to develop individualized treatment for endometrial cancer patients and further study Provide basis for diagnosis and treatment.