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采门诊男性急性淋病患者的脓性分泌物,经不同温度存放后,进行培养以观察其存活时间。所用培养基的配方如下:磷酸氢二钾2 g,磷酸二氢钾0.5 g,氯化钠2.5g,(月示)胨8 g,酵母膏3 g,玉米粉0.5 g,琼脂粉6 g,牛肉汤200 ml,50％卵黄液60ml,多粘菌素B 1.25万单位,万古霉素1500单位,蒸馏水250 ml。试验时,用沾有生理盐水的棉拭采取脓性分泌物,涂于纱布上。将经污染的纱布放在无菌平皿内,棉拭子放在无菌试管内,置
Patient outpatients with acute gonorrhea in patients with purulent secretions, after storage at different temperatures, to observe the survival time. The formulation of the medium used is as follows: dipotassium phosphate 2 g, potassium dihydrogen phosphate 0.5 g, sodium chloride 2.5 g, peptone 8 g, yeast extract 3 g, corn flour 0.5 g, agar powder 6 g, 200 ml of beef broth, 60 ml of 50% egg yolk liquid, 1.25 million units of polymyxin B, 1500 units of vancomycin and 250 ml of distilled water. Test, with cotton stained with saline to take purulent discharge, applied to the gauze. Put the contaminated gauze in a sterile dish and place the swab in a sterile tube