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建立HPLC方法测定犬血浆中的萘哌地尔浓度，研究萘哌地尔胶囊在犬体内的药物动力学。单剂量给予5只健康犬萘哌地尔胶囊200 mg，血浆样品碱化后，经乙醚提取以乙腈(磷酸盐缓冲液（0.05 mol(L-1 的磷酸二氢钾溶液，以0.1 mol(L-1的NaOH调节pH至6.5）= 60(40（v/v）为流动相，由ODS C18分析柱分离测定，紫外230 nm为检测波长，维拉帕米为内标。线性范围为10 ng(mL-1(1200 ng(mL-1；方法回收率为：100.23%(3.00%；检测限：8 ng(mL-1；日间RSD≤5.56%，日内RSD≤3.30%。本法简便，回收率和灵敏度高，可用于萘哌地尔制剂的药动学研究。单剂量给予犬萘哌地尔胶囊200 mg后血药浓度随时间变化规律符合一级吸收一室模型，T1/2Ke 为3.19(1.27 h, Tmax 为1.15(0.49 h, Cmax 为697.48(94.22 ng(mL-1。
To establish a HPLC method for the determination of the concentration of naftopidil in canine plasma and to study the pharmacokinetics of naftopidil in dogs. Five healthy dogs were given nafopidil capsules 200 mg in one single dose. The plasma samples were alkalified and extracted with ether (acetonitrile (phosphate buffer 0.05 mol (L-1, potassium dihydrogen phosphate solution, 0.1 mol (L -1 NaOH to adjust the pH to 6.5) = 60 (40 (v / v) as mobile phase, separated by ODS C18 analytical column with UV detection wavelength of 230 nm and verapamil as internal standard with a linear range of 10 ng (RSD≤5.56%, day RSD≤3.30%.) The method is simple and convenient, the method is simple, Recovery and high sensitivity can be used for pharmacokinetics study of naftopidil preparation.After a single dose of naftepilm capsule 200 mg of plasma concentration changes with time consistent with the first-order absorption model, T1 / 2Ke 3.19 (1.27 h, Tmax 1.15 (0.49 h, Cmax 697.48 (94.22 ng mL-1.