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目的 研究小檗碱对急性分离大鼠心室肌细胞内游离钙离子浓度 ([Ca2 + ] i)的影响。方法 采用酶解法分离单个大鼠心肌细胞 ,用钙敏感的荧光指示剂Fluo 3/AM染色 ,以荧光强度 (FI)来代表 [Ca2 + ] i,应用激光扫描共聚焦显微镜实时监测FI的变化。结果 在静息状态下 ,小檗碱 (3～ 1 0 0 μmol/L)对 [Ca2 + ] i 无影响。 3～ 1 0 0 μmol/L小檗碱对KCl 60mmol/L介导的钙内流也无影响。但小檗碱 (30和 1 0 0 μmol/L)对caffeine 1 0mmol/L引起的钙动员有明显促进作用 (P <0 .0 1 )。结论 小檗碱对于KCl通过电压依赖性钙通道 (VDC)介导的 [Ca2 + ] i 升高无影响 ;但小檗碱 (30和 1 0 0 μmol/L)可能通过影响RyRs而促进肌浆网 (SR)内钙外流 ,也可能对SR钙摄取 ,钙的跨膜转运及Na+ Ca2 + 交换有抑制作用
Objective To investigate the effect of berberine on intracellular free calcium concentration ([Ca2 +] i) in acutely isolated rat ventricular myocytes. Methods Single rat cardiomyocytes were isolated by enzymatic method and stained with calcium-sensitive fluorescent indicator Fluo 3 / AM. The fluorescence intensity (FI) was used to represent [Ca 2+] i. Real-time monitoring of FI was performed by laser scanning confocal microscopy. Results At rest, berberine (3 ~ 100 μmol / L) had no effect on [Ca2 +] i. Berberine concentration of 3 ~ 100 μmol / L had no effect on KCl 60 mmol / L-mediated calcium influx. But berberine (30 and 100 μmol / L) significantly promoted the calcium mobilization induced by Caffeine 10 mmol / L (P <0.01). Conclusions Berberine has no effect on the increase of [Ca2 +] i induced by KCl via voltage-dependent calcium channel (VDC). However, berberine (30 and 100 μmol / L) may promote sarcoplasmic reticulum Intrauterine (SR) calcium outflow may also have an inhibitory effect on SR calcium uptake, calcium transmembrane transport and Na + Ca2 + exchange