Three novel NEIL1 promoter polymorphisms in gastric cancer patients

• 作 者: Masanori Goto Kazuya Shinmura Shoichiro Tsugane Haruhiko Sugimura
• 机 构: First Department of Pathology,Hamamatsu University School of Medicine,1-20-1 Handayama,Higashi Ward,
• 出 处: World Journal of Gastrointestinal Oncology
• 发表日期: 2010年02期
• 关键词: promoter excision carcinogenesis repair allele strand sequencing subsequent iden

AIM:To identify genetic polymorphisms in the promoter region of the human base excision repair gene NEIL1 in gastric cancer patients.METHODS:The NEIL1 promoter region in DNA from 80 Japanese patients with gastric cancer was searched for genetic polymorphisms by polymerase chain reaction-single-strand conformation polymorphism and subsequent sequencing analyses.RESULTS:Three novel genetic polymorphisms,i.e.c.-3769C>T,c.-3170T>G,and c.-2681TA[8],were identified in the NEIL1 promoter region at an allele frequency of 0.6%,9.4%,and 4.4%,respectively,in Japanese gastric cancer patients.CONCLUSION:Three NEIL1 promoter polymorphisms detected in this study may be of importance in gastric carcinogenesis.

AIM: To identify genetic polymorphisms in the promoter region of the human base excision repair gene NEIL1 in gastric cancer patients. METHODS: The NEIL1 promoter region in DNA from 80 Japanese patients with gastric cancer was searched for genetic polymorphisms by polymerase chain reaction-single- strand conformation polymorphism and subsequent sequencing analyzes .RESULTS: Three novel genetic polymorphisms, iec-3769C> T, c.-3170T> G, and c.- 2681TA [8], were identified in the NEIL1 promoter region at an allele frequency of 0.6 %, 9.4%, and 4.4%, respectively, in Japanese gastric cancer patients. CONCLUSION: Three NEIL1 promoter polymorphisms detected in this study may be of importance in gastric carcinogenesis.