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目的：探讨抑癌基因ｐ５３与壶腹周围癌组织学分级和预后的关系。方法：用单克隆抗ｐ５３蛋白抗体（Ｄｏ－７）通过免疫组化ＬＳＡＢ法对６１例壶腹周围癌进行检测。结果：ｐ５３蛋白在壶腹周围癌中有较高的表达，阳性率为６７．２％（４１／６７）；组织学分级呈高分化组阳性率５１．７％（１５／２９），明显低于中低分化组阳性率８１．３％（２６／３２）（Ｐ＜０．０１）；术后３年生存率为４７．５％（２９／６１），其中术后３年内死亡组阳性率８４．４％（２７／３２），明显高于存活满３年组阳性率４８．３％（１４／２９）（Ｐ＜０．０１）。结论：ｐ５３蛋白的表达与壶腹周围癌的组织学分级成正相关而与术后生存期成负相关，提示ｐ５３蛋白的表达可作为判断壶腹周围癌组织学分级和预后的一个重要参考指标
Objective: To investigate the relationship between tumor suppressor gene p53 and histological grade and prognosis of periampullary carcinoma. Methods: 61 cases of periampullary carcinoma were detected by immunohistochemical LSAB method with monoclonal anti-p53 protein antibody (Do-7). Results: The p53 protein was highly expressed in periampullary carcinoma, the positive rate was 67.2% (41/67); the histological grade showed a positive rate of 51.7% (15/29) in the highly differentiated group. The positive rate in the moderately-differentiated group was 81.3% (26/32) (P<0.01); the 3-year survival rate was 47.5% (29/61) after surgery, among which the death rate was positive within 3 years after surgery. 84.4% (27/32) was significantly higher than the positive rate of 48.3% (14/29) in the 3-year survival group (P<0.01). Conclusion: The expression of p53 protein is positively correlated with the histological grade of periampullary carcinoma and negatively correlated with the postoperative survival, suggesting that the expression of p53 protein can be used as an important reference index to judge the histological grade and prognosis of periampullary carcinoma.