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继代１年以上的棉花品种珂字２０１下胚轴产生的胚性愈伤组织，转入加有不同浓度（０、８．５６×１０~（－２）、１．７１×１０~（－１）、２．５７×１０~（－１）、３．４２×１０~（－１）、５．１３×１０~（－４）、６．８４×１０~（－１）ｍｏｌ／Ｌ）ＮａＣｌ的筛选培养基上培养，经过３代筛选，获得耐盐胚性细胞系和再生植株。ＮａＣｌ显著抑制了愈伤组织的存活和生长，ＮａＣｌ半致死浓度在８．５６×１０~（－２）～１．７１×１０~（－１）ｍｏｌ／Ｌ之间，致死浓度为６．８４×１０~（－１）ｍｏｌ／Ｌ。ＮａＣｌ浓度影响着体细胞胚的发生和发育，影响着体细胞胚的萌发和植株再生。添加１．７１×１０~（－１）ｍｏｌ／ＬＮａＣｌ培养基，筛选出的愈伤组织生长较好，并能分化出体细胞胚和正常再生植株。在本实验中，笔者获得了耐３．４２×１０~（－１）ｍｏｌ／ＬＮａＣｌ的体细胞胚和耐１．７１×１０~（－１）ｍｏｌ／ＬＮａＣｌ的正常再生植株。
The embryogenic callus from the hypocotyls of the cotton cultivar “Kezi 201”, which inherited for more than 1 year, was transferred into the embryogenic callus with different concentrations (0, 8.56 × 10 -2, 1.71 × 10 ~ (- 1), 2.57 × 10 ~ (-1), 3.42 × 10 ~ (-1), 5.13 × 10 ~ (-4), 6.84 × 10 ~ (-1) mol / L) NaCl screening medium, after 3 generations of screening, salt-tolerant embryogenic cell lines and regenerated plants were obtained. NaCl significantly inhibited the survival and growth of callus. The lethal concentration of NaCl was between 8.56 × 10 -2 and 1.71 × 10 -1 mol / L, and the lethal concentration was 6.84 × 10 ~ (-1) mol / L. NaCl concentration affects the occurrence and development of somatic embryos, affecting the germination and plant regeneration of somatic embryos. Adding 1.71 × 10 ~ (-1) mol / L NaCl medium, the selected callus grew well and could differentiate into somatic embryos and normal regenerated plants. In this experiment, we obtained somatic embryos that were resistant to 3.42 × 10 ~ (-1) mol / L NaCl and normal regenerated plants that were resistant to 1.71 × 10 ~ (-1) mol / L NaCl.