论文部分内容阅读
Objective:To investigate the expressions of p53,RB1,Fas,c-fos,Ras,EGFR mRNA in human cervical cancer (HeLa)cell in response to the trichostatin A(TSA).Methods:We took count of HeLa cells in different incubation times with TSA(0.2μm/L).The result indicated that HeLa cells changed evidently when HeLa cells were incubated for 36 h.Then,we investigated the genes expression(mRNA levels)of HeLa cells after treatment for 36 h using SYBR green real-time PCR. Results:We demonstrated that trichostatin A(TSA)could make human cervical cancer(HeLa)cell morphological change and induce HeLa cell apoptosis.Furthermore,the data suggest that TSA-induced down-regulation of p53,RB1,Fas,but up- regulated c-fos gene expression after treatment for 36 h,and Ras,EGFR did not show obvious response to TSA treatments. Conclusion:TSA has different effects on gene expression.
Objective: To investigate the expressions of p53, RB1, Fas, c-fos, Ras, EGFR mRNA in human cervical cancer (HeLa) cell in response to the trichostatin A (TSA). Methods: We took count of HeLa cells in different incubation The results indicated that HeLa cells changed evidently when HeLa cells were incubated for 36 h. Then, we investigated the genes expression (mRNA levels) of HeLa cells after treatment for 36 h using SYBR green real time-PCR. Results: We demonstrated that TSA-induced make human cervical cancer (HeLa) cell morphological change and induce HeLa cell apoptosis. Future, the data suggest that TSA-induced down-regulation of p53, RB1, but up-regulated c-fos gene expression after treatment for 36 h, and Ras, EGFR did not show obvious response to TSA treatments. Conclusion: TSA has different effects on gene expression.