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目的：探讨肿瘤坏死因子－β（ＴＮＦ－β）对人胰腺癌ＪＦ３０５细胞增殖的抑制作用。方法：采用免疫细胞化学染色方法观察增殖细胞核抗原、ｂｃｌ－２蛋白的表达、３Ｈ－胸腺嘧啶核苷掺入经放射自显影显示ＤＮＡ合成、核仁组成区嗜银蛋白染色及末端脱氧核苷转移酶标记法凋亡原位染色，观察ＴＮＦ－β的作用。结果：ＰＣＮＡ及ｂｃｌ－２蛋白的表达，在ＴＮＦ－β处理前后变化不甚明显；ＡｇＮＯＲ染色后，银染颗粒的数量差异无统计学意义，但颗粒面积减少显著（Ｐ＜０．０５）；３Ｈ－胸腺嘧啶核苷放射自显影表明，Ｓ期细胞数明显减少，且差异显著。凋亡原位染色显示，在ＴＮＦ－β处理后的较短时间内即出现多量染色阳性细胞，与对照片比较，变化明显。结论：ＡｇＮＯＲ染色颗粒面积减少及凋亡原位染色出现多量凋亡细胞，能较敏感地反映ＴＮＦ－β处理对人胰腺癌细胞的增殖抑制作用。
Objective: To investigate the inhibitory effect of tumor necrosis factor-β (TNF-β) on the proliferation of human pancreatic cancer JF305 cells. METHODS: Immunocytochemical staining was used to observe the expression of proliferating cell nuclear antigen, bcl-2 protein, 3H-thymidine incorporation, DNA synthesis by autoradiography, argentin staining in nucleolar constitutive region, and terminal deoxynucleoside transfer. Apoptosis in situ staining with enzyme labeling was performed to observe the effect of TNF-β. Results: The expression of PCNA and bcl-2 protein did not change significantly before and after TNF-β treatment. There was no significant difference in the number of silver-stained particles after AgNOR staining, but the particle area decreased significantly (P<0.05). 3H-thymidine autoradiography showed that the number of cells in S phase was significantly reduced, and the difference was significant. Apoptotic in situ staining showed that a large number of positive cells were stained within a short time after TNF-β treatment, which was significantly different from the photos. Conclusion: The decreased area of AgNOR staining particles and the presence of apoptotic cells in situ in apoptotic cells can reflect the proliferation inhibition effect of TNF-β on human pancreatic cancer cells.