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With cell-mediated ~(51)Cr cytotoxicity assay,it was demonstratedthat the RNA extracted from H_(22) tumor cells(H_(22)TuRNA)could.significantly inhibit the cytotoxic effect of lymphocytes of normalmouse spleen when treated with H_(22)TuRNA at a dosage of≥250μg H_(22)TuRNA/1×10~7,lymphocytes at 37℃ for 60 min.The inten-sity of inhibition depended on the incubation time and the dose ofH_(22) TuRNA.The inhibition capacity was not abolished by treating with:RNase.Polyacrylamide gel electrophoresis showed that only thesmall molecules of RNA with the sedimentation rate about 4sremained after the treatment with RNase.Thus,it was consideredthat the RNA with small molecules which possesses anti-RNaseproperty was one of the active components in H_(22)TuRNA.H_(22)TuRNA could also inhibit the ability of lymphocytes toadhere to glass surfaces.This suggested that the function of H_(22)-TuRNA to inhibit the cytotoxic effect of lymphocytes was to inter-fere with the contact between lymphocytes and target cells.
With cell-mediated ~ (51) Cr cytotoxicity assay, it was demonstrated that RNA extracted from H_ (22) tumor cells (H_ (22) TuRNA) could.significantly inhibit the cytotoxic effect of lymphocytes of normal mouse spleen when treated with H_ ) TuRNA at a dosage of ≥ 250 μg H_ (22) TuRNA / 1x10 ~ 7, lymphocytes at 37 ° C for 60 min. In in-sity of inhibition depended on the incubation time and the dose of H_ (22) TuRNA. Inhibition capacity was not abolished by treating with: RNase. Polyacrylamide gel electrophoresis showed that only the molecules of RNA with the sedimentation rate about 4 sremained after the treatment with RNase.Thus, it was considered that the RNA with small molecules which possesses anti-RNase property was one of the active components in H_ (22) TuRNA.H_ (22) TuRNA could also inhibit the ability of lymphocytes toadhere to glass surfaces. This suggested that the function of H_ (22) -TuRNA to inhibit the cytotoxic effect of lymphocytes was to inter- fere with the contact between lymphocytes a nd target cells.