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目的:建立紫外线UVA(辐照强度为3650μJ·cm~(-2)对Hela细胞氧化损伤模型.探究扇贝多肽(PCF)对Hela细胞紫外线UVA氧化损伤的保护作用.方法:MTT法测定细胞活性;酶法测定抗氧化酶(GSH-Px、CAT、SOD)活性;流式细胞仪AnnexinV法测定细胞的凋亡率和死亡率;Fluo-3 AM为荧光染料,流式细胞仪测定细胞内游离Ca~(2+)的含量.结果:PCF(0.5％-2％)能明显增加 Hela细胞的增殖活性和细胞内游离Ca~(2+)的浓度.显著提高Hela细胞GSH-Px、CAT、SOD活性,且呈量效关系.同时降低Hela细胞的凋亡率和死亡率.PCF组与模型对照组比较各项指标均有统计学意义(P< 0.0 5,P<0.01).结论:扇贝多肽具有抗紫外线UVA对Hela细胞氧化损伤的作用.其机制与扇贝多肽提高抗氧化酶含量,抑制脂质过氧化有关.
OBJECTIVE: To establish a model of oxidative damage of Hela cells irradiated by UVA (irradiation intensity of 3650μJ · cm -2), and to explore the protective effect of scallop polypeptide (PCF) on UVA-induced oxidative damage of Hela cells.Methods: The activities of antioxidant enzymes (GSH-Px, CAT and SOD) were measured by enzyme-linked immunosorbent assay (ELISA). The apoptosis rate and mortality of cells were determined by flow cytometry AnnexinV method. Fluo-3 AM Results: PCF (0.5% -2%) significantly increased the proliferation activity of Hela cells and intracellular free Ca ~ (2+) concentration, and significantly increased the contents of GSH-Px, CAT and SOD (P <0.05, P <0.01) .Conclusion: Scallop polypeptide UVA has the effect of UVA on Hela cell oxidative injury.The mechanism is related to scallop peptides to increase antioxidant enzyme content and inhibit lipid peroxidation.