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目的观察舒血宁(SXN)对糖尿病SD大鼠肾脏的影响并探讨其可能机制。方法选取SPF级雄性SD大鼠腹腔注射STZ制备糖尿病模型,不同剂量SXN干预后,检测体重、血糖和24 h尿蛋白含量,Scr、BUN的变化,检测肾脏组织丙二醛(MDA)、超氧化物歧化酶(SOD)的水平。HE染色观察肾脏组织形态学改变,Western blot检测肾脏组织中TNF-α蛋白表达。结果大剂量SXN(12 mg/kg)治疗组(HSXN组)可明显逆转血清中Scr、BUN升高,逆转MDA的产生并升高SOD活性,减少24 h尿蛋白含量,增加体重和抑制肾脏中TNF-α的蛋白表达,而小剂量SXN(3 mg/kg)治疗组(LSXN组)仅抑制TNF-α的蛋白表达。结论SXN对DN具有一定的保护作用,其机制可能与抑制氧化应激和TNF-α表达有关。
Objective To observe the effects of Shuxuening (SXN) on the kidneys of diabetic SD rats and to explore its possible mechanism. Methods SPF male Sprague-Dawley rats were injected intraperitoneally with streptozotocin (STZ) to establish a diabetic model. After intervention with different doses of SXN, the changes of body weight, blood glucose, 24 h urinary protein, Scr and BUN were measured. Malondialdehyde (MDA) The level of dismutase (SOD). The morphological changes of kidney were observed by HE staining. The expression of TNF-α protein in kidney was detected by Western blot. Results High-dose SXN (12 mg / kg) treatment group (HSXN group) could significantly reverse the increase of serum Scr and BUN, reverse the production of MDA and increase the activity of SOD, reduce the content of 24 h urine protein, increase body weight and inhibit renal TNF-α protein expression, while low-dose SXN (3 mg / kg) treatment group (LSXN group) only inhibit TNF-α protein expression. Conclusion SXN may have a protective effect on DN, which may be related to the inhibition of oxidative stress and TNF-α expression.