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目的了解福建省流行的麻疹野病毒基因型别及特征。方法应用B95a细胞[Epstein-Barr(EB)Virus-Transformed,Marmoset B Lymphoblastoid Cell Line,埃泼斯坦-巴尔病毒转化的绒猴淋巴母细胞]或Vero/SLAM细胞[Vero Cells Transfected to Express the Human Signaling Lymphocyte Activation Molecule(SLAM),淋巴信号激活因子转染的非洲绿猴肾细胞],从疑似麻疹病例的咽拭子或尿液标本分离麻疹病毒,用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)扩增分离株病毒的核蛋白(Nucleoprotein,N)基因3’端450个核苷酸片段,分析其基因型别及遗传特征。结果 2002年、2006～2009年,共从6个设区的市分离了24株麻疹野病毒,均为H1基因型、H1a基因亚型,未分离到其他型别的毒株。福建株间核苷酸同源性为97.8%～100.0%,在遗传树图中属于4个独立分支谱系(Lineage),Lineage4分支病毒出现了3个新的氨基酸突变位点。福建株中来自不同地区和年份的MV(Measles Virus,麻疹病毒)07-29FZ(Fuzhou,福州)、MV07-20ND(Ningde,宁德)、MV07-47ND和MV08-78QZ(Quanzhou,泉州)、MV06-32LY(Longyan,龙岩)、MV07-30FZ、MV07-39FZ和MV07-46ND的N基因3’端的450核苷酸同源性均为100.0%。福建株与H1基因型中国代表株、H2基因型及疫苗株S(Shanghai,上海)191相比,与S191疫苗株的A基因组差异最大(核苷酸同源性90.1%～93.3%,氨基酸同源性86.8%～91.4%)。结论 H1a为福建省本土麻疹野病毒优势基因亚型,未发现其他基因亚型。近3年的分离株出现了新的氨基酸位点突变。不同地区或同一地区内存在多传播链同时循环,也存在同一野病毒株在不同地区、不同年份持续循环。
Objective To understand the genotypes and characteristics of wild measles virus in Fujian Province. Methods B95a cells [Epstein-Barr (EB) Virus-Transformed, Marmoset B Lymphoblastoid Cell Line, or Vero / SLAM cells transformed with Epstein-Barr virus [Vero Cells Transfected to Express the Human Signaling Lymphocyte Activation Molecule (SLAM), a lymphokine-activated green monkey kidney cell, was isolated from throat swabs or urine samples from suspected measles cases. Measles virus was detected by Reverse Transcription-Polymerase Chain PCR was used to amplify the 450 nucleotide fragments of the 3 ’end of the nucleoprotein (N) gene of the virus isolated from the virus. The genotypes and genetic characteristics were analyzed. Results In 2002 and 2006-2009, a total of 24 wild measles virus strains were isolated from 6 districts, which were all H1 and H1 subtype. No other strains were isolated. The nucleotide homology between Fujian isolates was 97.8% -100.0%, belonging to 4 independent lineages in the genetic tree map, and 3 new amino acid mutation sites were found in Lineage 4 branch viruses. MV (Measles Virus) 07-29FZ (Fuzhou, Fuzhou), MV07-20ND (Ningde), MV07-47ND and MV08-78QZ (Quanzhou, Quanzhou) from different regions and years in Fujian Province, MV06- The 450 nucleotide homology of the 3 ’end of the N gene of 32LY (Longyan, Longyan), MV07-30FZ, MV07-39FZ and MV07-46ND was 100.0%. Compared with the H1 representative Chinese strain, the H2 genotype and the vaccine strain S (Shanghai, Shanghai) 191, the Fujian strain showed the greatest difference from the A genome of the S191 vaccine strain (nucleotide identity 90.1% -93.3%, amino acid identity Source 86.8% ~ 91.4%). Conclusion H1a is the predominant genotype of measles wild virus in Fujian Province, and no other gene subtypes were found. Nearly 3 years of isolates showed a new amino acid site mutation. In different regions or in the same area there is a multi-propagation chain circulating at the same time, there are also the same wild virus strains in different regions and different years of continuous cycle.