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目的:建立测定人体血浆中喷昔洛韦高效液相色谱法,并测定健康志愿者口服泛昔洛韦片12h内血药浓度。方法:采用6%高氯酸直接沉淀提取血浆,色谱柱为C18(250mm×4.6mm,5μm),0.4%磷酸-甲醇(95∶5)为流动相,流速0.8mL·min-1,荧光检测波长为λex=270nm,λem=375nm。结果:喷昔洛韦本法线性范围为0.05～5μg·mL-1(r=0.9999,n=7);最低定量限0.05μg·mL-1;低、中、高3种浓度的平均回收率分别为(97.80±2.28)%,(96.60±0.37)%,(96.58±0.38)%;日内、日间精密度RSD均<4%。结论:本检测方法简便、快速、经济、准确,可用于测定人血浆中喷昔洛韦的浓度。
OBJECTIVE: To establish a HPLC method for the determination of penciclovir in human plasma and to determine the plasma concentration of famciclovir tablets within 12 hours in healthy volunteers. Methods: The plasma was precipitated directly by 6% perchloric acid. The chromatographic column was C18 (250mm × 4.6mm, 5μm) with a mobile phase of 0.4% phosphoric acid-methanol (95: 5) at a flow rate of 0.8mL · min- The wavelengths are λex = 270 nm, λem = 375 nm. Results: The linear range of this method was 0.05 ~ 5μg · mL-1 (r = 0.9999, n = 7) and the lowest limit of quantitation was 0.05μg · mL-1. The average recoveries of low, medium and high concentrations of penciclovir were (97.80 ± 2.28)%, (96.60 ± 0.37)% and (96.58 ± 0.38)%, respectively. The intra-day and inter-day RSD were all less than 4%. Conclusion: The method is simple, rapid, economical and accurate and can be used to determine the concentration of penciclovir in human plasma.