论文部分内容阅读
目的:探讨血竭含药血清对皮肤成纤维细胞TGF-β1/Smad3信号通路的影响。方法:将大鼠随机分为血竭高、中、低剂量含药血清组和正常对照组,每组6只。用药组分别以0.2、0.1、0.05 g·kg-1·d-1的血竭灌胃,正常对照组给予生理盐水,7 d后取血制备含药血清。组织块法培养大鼠成纤维细胞,检测血竭含药血清对成纤维细胞增殖活性、TGF-β1分泌水平以及TGF-βRⅠ、TGF-βRⅡ、Smad3和p-Smad3蛋白表达的影响。结果:与正常对照组比较,血竭各剂量含药血清能显著提高成纤维细胞的增殖活性和TGF-βRⅠ、TGF-βRⅡ及p-Smad3蛋白的表达(P<0.05或P<0.01);中、高剂量含药血清组TGF-β1水平、Smad3、和Smad3蛋白磷酸化水平明显上升(P<0.05或P<0.01)。结论:血竭对创伤的修复作用可能与其调节TGF-β1/Smad3信号转导通路有关。
OBJECTIVE: To investigate the effect of serum-containing serum of Radix Xizi on TGF-β1 / Smad3 signaling pathway in human dermal fibroblasts. Methods: The rats were randomly divided into high, medium and low dose serum containing serum group and normal control group, 6 rats in each group. The rats in the drug-treated group were orally administered with 0.2, 0.1 and 0.05 g · kg-1 · d-1 of blood serum respectively. The normal control group was given normal saline, and the blood was collected 7 days later to prepare the drug-containing serum. The rat fibroblasts were cultured by tissue block method. The effects of serum-containing serum of Radix astragali on fibroblast proliferation, secretion of TGF-β1 and the expression of TGF-βRⅠ, TGF-βRⅡ, Smad3 and p-Smad3 were detected. Results: Compared with the normal control group, serum-containing serum of each dose of Radix Serum could significantly increase the proliferation activity and the expression of TGF-βRⅠ, TGF-βRⅡ and p-Smad3 in fibroblasts (P <0.05 or P <0.01) (P <0.05 or P <0.01). The levels of TGF-β1, Smad3, and Smad3 in the high-dose group were significantly increased (P <0.05 or P <0.01). Conclusion: The repair effect of blood serum on trauma may be related to its regulation of TGF-β1 / Smad3 signal transduction pathway.