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[目的]应用单核细胞THP-1株建立单核细胞向巨噬细胞分化的体外模型,为巨噬细胞介导的慢性炎症在颈椎病的发生发展机制及其防治提供基础。[方法]用6 ug/L乙酸豆寇佛波酯(PMA)诱导THP-1细胞48 h向成熟的巨噬细胞分化后,采用倒置显微镜观察分化细胞的形态学变化。利用流式细胞术测定巨噬细胞表面特异性抗原F4/80、CD14,验证PMA-THP-1诱导效果。[结果]经PMA诱导后,THP-1细胞由类圆形、分散、悬浮转变为不规则、黏附、贴壁,且出现F4/80、CD14的高表达。[结论]从离体细胞培养方面建立并验证了人单核源性巨噬细胞的模型,该模型材料来源广、制备简单,并可用作巨噬细胞与颈椎病发生发展关系的研究。
[Objective] To establish monocyte-macrophage differentiation model in vitro by monocyte THP-1 strain and provide the basis for the mechanism of macrophage-mediated chronic inflammation in the pathogenesis and prevention of cervical spondylosis. [Method] THP-1 cells were induced to differentiate into mature macrophages 48 h after treatment with 6 ug / L acetic acid myrothecium (PMA), and morphological changes of differentiated cells were observed by inverted microscope. Flow cytometry was used to determine the surface antigen-specific macrophages F4 / 80 and CD14 to verify the induction effect of PMA-THP-1. [Results] After induced by PMA, THP-1 cells changed from round, scattered and suspended to irregular, adherent and adherent, with high expression of F4 / 80 and CD14. [Conclusion] The model of human monocyte-derived macrophages was established and verified from in vitro cell culture. The material of this model is widely originated and simple to prepare. It can be used as a study of the relationship between macrophages and cervical spondylosis.