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目的探讨全反式维甲酸(ATRA)对人雄激素非依赖性前列腺癌细胞株PC-3增殖的抑制作用及其Bcl-2和Bax表达的影响。方法以不同浓度的ATRA处理PC-3细胞,甲基偶氮唑蓝(MTT)比色法检测细胞增殖抑制情况;流式细胞仪和免疫细胞化学法检测PC-3细胞Bax和Bcl-2的表达。结果ATRA浓度为(10-6~10-4)mol/L时,可明显抑制PC-3细胞的增殖(P<0.01),并具有时间-剂量依赖性。ATRA处理后PC-3细胞Bcl-2表达下调,Bax表达上调。随ATRA浓度增加,Bcl-2/Bax的比值不同程度地下降(P<0.05)。结论ATRA对PC-3细胞具有明显的增殖抑制作用,并且与ATRA下调Bcl-2表达、上调Bax表达有关。
Objective To investigate the effect of all-trans retinoic acid (ATRA) on the proliferation of human androgen-independent prostate cancer cell line PC-3 and the expression of Bcl-2 and Bax. Methods PC-3 cells were treated with different concentrations of ATRA, and MTT assay was used to detect the cell proliferation inhibition. Flow cytometry and immunocytochemistry were used to detect the expression of Bax and Bcl-2 in PC-3 cells expression. Results ATRA concentration of (10-6 ~ 10-4) mol / L significantly inhibited the proliferation of PC-3 cells (P <0.01) in a time-and dose-dependent manner. After ATRA treatment, Bcl-2 expression was down-regulated and Bax expression was up-regulated in PC-3 cells. With the increase of ATRA concentration, the ratio of Bcl-2 / Bax decreased to some extent (P <0.05). Conclusion ATRA can significantly inhibit the proliferation of PC-3 cells, and may be related to the down-regulation of Bcl-2 expression and up-regulation of Bax expression by ATRA.