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午毒蛾是世界性的大害虫,分布广,食性杂。在我国主要危害,杨、柳、桦、柞、落叶松、油杉及果树等多种针阔叶树种,它是我国林业、蚕业、果树及农业的主要害虫之一。 一九七六年至一九七七年,我们先后于辽宁省庄河,沈阳市发现了午毒蛾NPV。两年来,对午毒蛾的NPV的多角体,病毒束及病毒粒子等形态结构作了电镜观察,其结果是,多角体为六面体或不规则形,每多角体内包藏着70～80条病毒束,每病毒束包藏着1～14条或更多的病毒粒子。病毒粒子为杆状,释放后,略呈指状弯曲。我们完成了午毒蛾NPV对午毒蛾室内外毒力测定,其结果是:室外大面积防治是在辽宁省盖县塔寺大队120亩柞林内进行的,用8.2×10~5PIBs/ml的浓度对Ⅰ—Ⅳ令幼虫进行毒力测定,其结果是Ⅰ令幼虫.LC_5 2×10~2PIBs/ml LT_(50)11天;Ⅱ令幼虫LC_(50)1×10~5PIBs/ml,LT_(50)10天,Ⅲ令幼虫LC_(50)2×10~5PIBs/ml,LT_(50)8天;Ⅳ令幼虫LC_(50)2×10~7PIBs/ml,LT_(50)10.5天。两年来对柞蚕经过多次反复喂饲多角体,并用午毒蛾病毒蛹的 体液注射柞蚕的蚕蛹子,结果表明均完全无害,并能在自然界形成流行病的高毒力病毒。
Gypsy moth is a worldwide pest, widely distributed, eating complex. In our country, the main hazards are poplar, willow, birch, oak, larch, oleander and fruit trees and other coniferous and broadleaf species. It is one of the major pests of forestry, sericulture, fruit trees and agriculture in our country. From 1976 to 1977, we successively found NPV in China in Zhuanghe and Shenyang in Liaoning Province. In the past two years, the morphological structures of NPV polyhedrosis, virus bundles and virions of H. guttatum were observed electron microscopically. As a result, polyhedra were hexahedron or irregular with 70-80 virus bundles in each polyhedron, Each virus bundle contains 1 to 14 or more virions. Virus particles for the rod, after release, slightly finger-bending. We completed the H. gibexate NPV indoor and outdoor toxicity determination of H. guttatus, the result is: outdoor large-scale prevention and control is in Liaoning Province Gaoxian Pagoda 120 acres of oak forest, with 8.2 × 10 ~ 5PIBs / ml concentration of Ⅰ-Ⅳ larvae for virulence determination, the result is Ⅰ larvae .LC_5 2 × 10 ~ 2PIBs / ml LT_ (50) 11 days; Ⅱ larvae LC_ (50) 1 × 10 ~ 5PIBs / ml, LT_ ) For 10 days, and for larvae LC_ (50) 2 × 10 ~ 5PIBs / ml for Ⅲ and 8 days for LT_ (50). Two years of tussah after repeated feeding of polyhedra, and homing moth virus pupa body fluid injection silkworm pupae, the results show that are completely harmless and can form a high prevalence of virulent viruses in nature.