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目的 阐明Hes5通过调节哪些蛋白的表达而抑制神经细胞的分化.方法 利用蛋白组学同位素标记相对和绝对定量技术(iTRAQ技术)观察Hes5敲除生后8d小鼠室下带蛋白表达的变化,并用Western blotting技术对表达上调和下调的蛋白进行验证.结果 在Hes5敲除生后8d小鼠室下带发现19种蛋白有重要的变化.与对照组野生型小鼠相比,其中10种蛋白的表达是上调的,变化的倍率在1.20～1.32之间.9种蛋白的表达是下调的,变化的倍率在0.77～0.83之间.上调的蛋白主要与促进神经细胞的生长,细胞的迁移,黏附和信号的转导有关,而下调的蛋白主要是抑制细胞的生长和维持细胞骨架的蛋白.Westem blotting验证蛋白表达的变化与iTRAQ结果一致.结论 Hess可能通过影响与细胞生长、迁移以及细胞骨架等有关的多种蛋白的表达来影响细胞的分化.“,”Objective Hes5 is one of the critical effectors of the Notch pathway and its expression Call inhibit neuronal differentiation.Which protein expression is influenced in cell differentiation by HeS5 remains to be determined.Methods Experiments were designed to study changes in protein expression in the subventricular zone(SVZ)of the HesS knockout mice at the postnatal day 8 using the new method of proteomic isobaric tags for relative and absolute quantification(ITRAQ),and confirmed expression of the up-gnlated and down-regulated proteins by Western blotting.Results Nineteen proteins showed differences in expression levels in the SVZ of Hes5 knockout mice.When compared tO the wild type mice,expressions of 11 proteins in the knockout mice were up-regulated,and the differentially expressed proteins showed ratios of knockout/widtype between 1.20 and 1.32.Expressions of 9 proteins were down-regulated,with their ratios were between 0.77 and 0.83.The upregulated proteins have been shown to be involved in neurogenesis,cell adhesion,migration and signal transduction.The downregulated proteins have been mainly shown to be involved in growth inhibition and cytoskeleton.Westem bloning analysis further confirmed the expression of proteins from iTRAQ.Conclusion Our findings suggest that Hes5 may influence cell differentiation by regulating expression of some proteins involved in cell growth and migration,and its cytoskeleton.