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目的探讨T-2毒素对大鼠脑组织脂质过氧化作用及其与低硒因素的协同作用。方法选用新生断乳SD大鼠,随机分为基础组和低硒组。低硒动物模型建立成功后,将基础组随机分为基础组、低T-2组、高T-2组,低硒模型组随机分为低硒组、低硒+低T-2组、低硒+高T-2组,每天以0.1 mg/kg BW为低T-2剂量和0.2 mg/kg BW为高T-2剂量灌胃染毒1个月。染毒月末将大鼠断头处死,取全脑。作病理切片,观察大鼠神经细胞变化;测定脑组织中GSH-Px活性和MDA含量。结果低硒各组大鼠脑组织中GSH-Px活性均显著低于基础组(P<0.05);各组大鼠脑组织中MDA含量均显著高于基础组(P<0.05)。结论 T-2毒素能够抑制GSH-Px活性,引起MDA明显增多,并与低硒因素有协同作用。
Objective To investigate the synergistic effect of T-2 toxin on lipid peroxidation and low selenium in rat brain. Methods Neonatal weaning SD rats were randomly divided into basic group and low-selenium group. Low selenium group, low selenium group and low T-2 group were randomly divided into basal group, low T-2 group, high T-2 group and low selenium group Selenium + high T-2 group. The mice were given intragastric administration of 0.1 mg / kg BW as a low T-2 dose and 0.2 mg / kg BW as a high T-2 dose for one month. The rats were sacrificed at the end of the month of exposure and the whole brain was taken. Pathological sections were made to observe the changes of nerve cells in rats. The activity of GSH-Px and the content of MDA in brain tissue were determined. Results The activity of GSH-Px in brain tissues of rats in low selenium group was significantly lower than that in basal group (P <0.05). The content of MDA in brain tissue of rats in each group was significantly higher than that in basal group (P <0.05). Conclusion T-2 toxin can inhibit the activity of GSH-Px, cause a significant increase of MDA and synergistic effect with low selenium.