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在初步用计算机辅助的羧肽酶法测定了天花粉蛋白 C-端顺序的基础上,进一步设计了两个计算机程序——DPS 程序和 CPA 程序.用合成小肽和天然的肽对这两个计算机程序进行模型实验证明,运用这两个程序能分别满意地从羧肽酶的酶解动力学曲线中获得重要的 C-端顺序信息,并测定了天花粉蛋白分子中未知肽段 CB-3的 C-端顺序为:—SerAlaSerAlaAlaLeuHserOH,这一顺序后来已经其他实验结果所证实.本法不仅使 C-端顺序测定延长至七个氨基酸,而且还基本上解决了多肽或蛋白质含有多种多次重复氨基酸残基的 C-端顺序测定.
Based on the preliminary computer-assisted carboxypeptidase method for the determination of the C-terminal sequence of Trichosanthin, two computer programs, the DPS program and the CPA program, were further designed. Both the synthetic computer and natural peptide The results of the model experiments show that using these two procedures, we can obtain the important C-terminal sequence information from the enzymatic hydrolysis kinetics curve of carboxypeptidase, respectively, and determine the C - end sequence: -SerAlaSerAlaAlaLeuHserOH, this sequence has been confirmed by other experimental results.This method not only to C-terminal sequencing extended to seven amino acids, but also basically solved the polypeptide or protein contains a variety of multiple repeat amino acids The C-terminal sequence was determined.