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耐药性艾滋病病毒的出现大大增加了高效抗逆转录病毒治疗(HAART)失败的几率。为探究病毒底层耐药规律,近年来以耐药相关蛋白为切入点,利用X线晶体衍射、NMR解析和生物信息学技术中的生物建模和分子动力学等方法探究病毒耐药相关蛋白结构变异方面的研究日益成为人们关注的热点,其研究成果为理解耐药毒株的耐药机制提供了新的依据。本文就近年来利用上述方法获得的HIV-1耐药相关蛋白-逆转录酶的耐药变异情况进行简要综述,以期为临床合理用药和新药开发提供指导。
The emergence of drug-resistant HIV greatly increases the chances of failing highly active antiretroviral therapy (HAART). In order to explore the rule of drug resistance in the bottom of the virus, in recent years, the drug resistance-related protein as the starting point, the use of X-ray crystal diffraction, NMR analysis and bioinformatics techniques in biological modeling and molecular dynamics methods to explore the virus resistance-related protein structure Mutation research is increasingly becoming a hot topic of concern, and its research provides a new basis for understanding the drug resistance mechanism of drug-resistant strains. In this paper, we briefly reviewed the resistance variation of HIV-1 resistance-related protein-reverse transcriptase obtained by the above methods in recent years, in order to provide guidance for the rational use of drugs and the development of new drugs.