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以西瓜种内的１２个品种（系）为模板ＤＮＡ，研究了影响ＲＡＰＤ扩增效果的因素。结果表明，模板ＤＮＡ、Ｔａｑ酶、随机引物、Ｍｇ２＋浓度以及ＰＣＲ反应程序在ＲＡＰＤ分析中都具有重要的作用。根据以上因子的研究结果，建立了适合西瓜种植物的ＲＡＰＤ分析的技术体系：１０μＬ反应液中，模板１０ｎｇ／１０μＬ，引物０５μｍｏｌ／Ｌ，ＴｒｉｓＨＣｌ（ｐＨ８３）５０ｍｍｏｌ／Ｌ，ＢＳＡ５００μｇ／ｍＬ，ＭｇＣｌ２２５ｍｍｏｌ／Ｌ，ＴａｑＤＮＡ０６Ｕ／１０μＬ，ｄＮＴＰｓ２００μｍｏｌ／Ｌ，１０％蔗糖４００和１ｍｍｏｌ／Ｌ甲酚红。
Twelve varieties (lines) of watermelon species were used as template DNA to study the factors influencing the amplification effect of RAPD. The results showed that the template DNA, Taq enzyme, random primer, Mg2 + concentration and PCR reaction procedure all play an important role in RAPD analysis. According to the above research results, the technical system of RAPD analysis for watermelon seeds was established: 10ng / 10μL of template, 05μmol / L of primer, 50mmol / L of TrisHCl (pH83), 500μg of BSA / ML, MgCl22.5 mmol / L, Taq DNA 0.6 U / 10 μL, dNTPs 200 μmol / L, 10% sucrose 400 and 1 mmol / L cresol red.