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本实验用[~3H]-脱氧葡萄糖自显影术研究纳洛酮对电针“足三里”穴区传入信息在脊髓、脑干中传递的影响。实验结果用日本MPM-2型光密度微测仪测量各核团自显影象的光密度值(D)。结果表明:(1)电针左侧“足三里”穴区可引起注射了纳洛酮(125μg/0.2ml,iv)的大鼠(n=8)同侧腰髓背角自显影象的光密度增加[电针侧(左侧)背角光密度值平均为0.689±0.026D,非电针侧(右侧)为0.498±0.033D](P<0.05)。其中5只大鼠左侧薄束核自显影象的光密度不增加,此时牵拉胃可引起连合核自显影象的光密度增加(较对照点平均增加37.82±7.07％,P<0.05);反之,另8只大鼠针刺侧薄束核自显影象的光密度呈现增加(较非针刺侧薄束核平均增加58.2±17.09％,P<0.05),这时牵拉胃未能引
In this study, [~ 3H] -deoxyglucose autoradiography was used to study the effect of naloxone on the transmission of afferent information in the “Zusanli” acupuncture points in the spinal cord and brain stem. Experimental results The optical density value (D) of the self-developed image of each nucleus was measured with a Japan MPM-2 optical density micro-meter. The results showed that: (1) The “Zusanli” acupuncture area on the left side of electroacupuncture could induce the spontaneous imaging of ipsilateral lumbar dorsal horn of rats (n = 8) injected with naloxone (125μg / 0.2ml, iv) The increase in optical density (average optical density at the electroacupuncture (left) dorsal angle of 0.689 ± 0.026D and at the non-electroacupuncture side (right) was 0.498 ± 0.033D] (P <0.05). The optical density of self-imaging of the left bundle side of 5 rats did not increase, at this time, the optical density of self-imaging of the conjunctival nuclear increased (compared with the control point average increase of 37.82 ± 7.07%, P <0.05). On the contrary, the optical densities of the spontaneous visualization of the other side of the acupuncture-side thin-stranded nuclei increased (by an average of 58.2 ± 17.09%, P <0.05) compared with that of the non-acupuncture side Pull the stomach failed to lead