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目的:探讨了华蟾毒精、远华蟾毒精、沙蟾毒精和杠柳苷4种强心苷类成分的镇痛作用及华蟾毒精的镇痛作用机制。方法:昆明种小鼠按要求分为空白组,阿司匹林阳性组(200 mg·kg-1),各受试化合物高、中、低剂量组,采用小鼠热板法和醋酸扭体法观察4种强心苷类化合物的镇痛作用;通过注射盐酸纳洛酮(2 mg·kg-1)和外周阿片受体阻断剂(纳洛酮四价盐衍生物,NAL-M,20 mg·kg-1)初步研究华蟾毒精的镇痛作用机制。结果:与空白组比较,华蟾毒精、远华蟾毒精、沙蟾毒精和杠柳苷均能提高热板所致小鼠舔足的痛阈(P<0.05,P<0.01),也能使醋酸所致小鼠扭体次数减少(P<0.05,P<0.01);ip纳洛酮和NAL-M后,均能拮抗华蟾毒精对热板所致小鼠痛阈值的提高作用(P<0.05,P<0.01)。结论:华蟾毒精、远华蟾毒精、沙蟾毒精和杠柳苷均具有镇痛作用,且华蟾毒精的镇痛作用与外周阿片受体有一定的关联。
OBJECTIVE: To investigate the analgesic effects of canadonin, farnesetin, resazurin and barium glycosides and the analgesic mechanism of bufalin. Methods: Kunming mice were divided into blank group, aspirin-positive group (200 mg · kg-1), high, medium and low doses of each test compound. The mice were treated with hot plate method and acetic acid writhing method. (Naloxone, tetravalent salt derivatives, NAL-M, 20 mg · kg-1) and injection of naloxone hydrochloride (2 mg · kg -1) and peripheral opioid receptor blockers kg-1) preliminary study of bufalin analgesic effect mechanism. Results: Compared with the blank group, bufalin, farnesetin, res toadine and barprinidin all increased the pain threshold of licking feet induced by hot plate in mice (P <0.05, P <0.01) (P <0.05, P <0.01). Both ip naloxone and NAL-M antagonized the increase of the pain threshold induced by hot plate (P <0.05, P <0.01). CONCLUSION: Both bufalin, farrein, res toadine and barprinoside all have analgesic effects. The analgesic effect of canadon toxin is related to peripheral opioid receptors.