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选择适当的蘖穗,在实验室迅速制片;或插入水中在室温条件下保存,不超过3小时.从新鲜穗上挑选大约在减数分裂时期的小花,每朵花取一个花药,用醋酸洋红制镜检临时片.一定要从大的小穗到小的小穗,从每个小穗最大的小花到最小的小花成熟顺序依次检察.将确认时期适合的小花中的其余两个花药取出,用新配的3份无水酒精加一份冰醋酸溶液固定,指管加塞子,贴上标签,在室温下至少放30分钟.然后在2℃下可存放4天.花药如需长期保存,可换入70%酒精在2℃下保存.
Select the appropriate tiller spike, in the laboratory rapid production; or insert into water at room temperature for less than 3 hours.From the fresh ear about the selection in the meiosis period florets, each flower to take an anther, with acetic acid Magenta Microscopy temporary film must be from the small spikelets to small spikelets, from each spikelets to the smallest floret the smallest mature order of the flowers in order to check the timing of the floret to remove the remaining two anthers , Fix it with 3 fresh parts of anhydrous alcohol plus 1 part of glacial acetic acid, add vial to the tube, label it and let it stand for at least 30 minutes at room temperature, then for 4 days at 2 ° C. , Can be exchanged into 70% alcohol stored at 2 ℃.