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肥大细胞的来源尚无定论,我们拟进行探索.阴栓龄14、15天的Wistar胎鼠,横断其胸腹部,整块固定于4%多聚甲醛液内过夜.17～20天胎鼠及生后1天者,直接取其右侧睾丸和胸腺.生后5、10、20及40天幼鼠先经心脏灌流上述固定液半小时后,再取其睾丸和胸腺固定于同液内过夜.动物均购自中国医学科学院实验动物中心.按常规石蜡包埋,切片5μm厚,脱蜡至70%酒精,0.2%甲苯胺兰(60%酒精配制)染1分钟,蒸馏水速洗,滤纸吸去水份,甲醇脱水2次,各2分钟,透明、封固、观察.结果:14～15天胚胎胸腹部各器官内间充质繁殖旺盛,有些部位细胞排列紧密,没有看到肥大细胞.胚胎17天时,胸腺已发育良好,小叶结构明显.小叶间隔结缔组织内出现数个肥大细胞,体积小呈椭圆形,核圆而谈染,呈浅蓝色,细胞质中含有深紫红色的圆形异染性颗粒.胚胎19天时,胸腺附近的骨骼肌结缔组织内也出现几个肥大细胞,而软骨膜上则没有.生后1天时,胸腺实质内开始出现同个体积小的肥大细胞,小叶间隔内的肥大细胞体积增大,数量增多,扩展至被膜下.其后至40天数量渐多.但同时期的睾丸内却没有肥大细胞的出现.本结果提示,肥大细胞先在中枢免疫器官——胸腺内出现,表示其与免疫作用关系密切,以后才在其他器官内陆续出现.在胸腺内先于小叶间隔,后于实质内出现.而与免疫无
Mast cells of the source is not conclusive, we intend to explore.Witharrow age 14,15 days of Wistar fetal rats, transecting the chest and abdomen, monolith fixed in 4% paraformaldehyde solution overnight .17 ~ 20 days of fetal rat and One day after birth, the right testes and thymus were taken directly.After 5, 10, 20 and 40 days of birth, the young rats were perfused with the cardiomyocytes by cardiac perfusion for half an hour, then the testis and thymus were fixed in the same liquid overnight Animals were purchased from the Chinese Academy of Medical Sciences Experimental Animal Center.Palconical paraffin embedded section 5μm thick, dewaxed to 70% alcohol, 0.2% toluidine blue (60% alcohol preparation) dyed 1 minute, washed with distilled water, filter paper suction The water and methanol were dehydrated twice for 2 minutes, respectively, and then transparent, sealed and observed.RESULTS: The interstitial and interstitial cells in the thorax and abdomen of 14-15 days embryos were exuded vigorously, and the cells in some areas were closely arranged with no mast cells. The thymus was developed well and the structure of the leaflets was obvious at 17th day of embryo.The number of mast cells appeared in the connective tissue of the interlobular septa.The small size was oval and the nucleus was round and talked with light blue.The cytoplasm contained a dark purple circle Heterogeneous particles. Embryos 19 days, the thymus near the skeletal muscle connective tissue also appeared several hypertrophy Cells, but not on the perichondrium.At 1 day after birth, the same small volume of mast cells started to appear in the thymus parenchyma, and the number of mast cells in the interlobular septa increased and expanded to the subfilm, and then to 40 days But the same period of testes but no mast cells appear.The results suggest that mast cells in the central immune organs first appeared in the thymus, indicating that its close relationship with the immune function, and later appeared in other organs Within the thymus before the interlobular septa, after the emergence of the substance, but with no immunity